| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blanchard, Makaila (13)
|
FGSF |
38.60 |
38.73 |
0.13 |
| 2 |
Bailey, Justine L (14)
|
FGCCPR |
41.86 |
40.18 |
-1.68 |
| 3 |
Cadavid, Nicole N (13)
|
FGUN-0 |
39.51 |
40.20 |
0.69 |
| 4 |
Mizrachi, Elleyah R (13)
|
FGPST |
40.74 |
40.35 |
-0.39 |
| 5 |
Parsotan, Nadia A (14)
|
FGPST |
38.79 |
41.23 |
2.44 |
| 6 |
Muravyeva, Arina (14)
|
FGHAT |
44.63 |
41.36 |
-3.27 |
| 7 |
Blanchard, Tamalia (13)
|
FGSF |
41.32 |
42.15 |
0.83 |
| 8 |
Schleicher, Carleigh M (13)
|
FGUN-0 |
43.38 |
42.68 |
-0.70 |
| 9 |
Wooden, Azana (14)
|
FGSFTL |
42.69 |
43.00 |
0.31 |
| 10 |
Wehby, Carly (13)
|
FGSUN |
41.97 |
43.79 |
1.82 |
| 11 |
Saballos, Fabiola (14)
|
FGPST |
42.78 |
44.43 |
1.65 |
| 12 |
Rozin, Carla B (13)
|
FGTSA |
45.65 |
45.63 |
-0.02 |
| 13 |
Juskiewicz, Abigail E (13)
|
FGPST |
43.87 |
46.42 |
2.55 |
| 14 |
Feudo, Jillian G (14)
|
FGPST |
48.62 |
49.43 |
0.81 |
|
Roberti, Isabella K (13)
|
FGAZFL |
|
DQ |
- |
|
Hernandez, Valeria (14)
|
FGYSF |
|
DQ |
- |
|
Jimenez, Victoria (13)
|
FGSFTL |
|
NS |
- |