| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nieto, Bianca G (12)
|
FGGRSC |
32.67 |
33.78 |
1.11 |
| 2 |
Rosenthal, Reese E (12)
|
FGGRSC |
33.75 |
34.93 |
1.18 |
| 3 |
Wood, Elyse A (12)
|
FGGRSC |
34.90 |
35.29 |
0.39 |
| 4 |
Negrin, Paola (12)
|
FGGRSC |
36.79 |
36.21 |
-0.58 |
| 5 |
Idler, Sahara (12)
|
FGMACM |
41.10 |
36.91 |
-4.19 |
| 6 |
Foster, Valentina (12)
|
FGMWA |
36.84 |
37.15 |
0.31 |
| 6 |
Milano, Mariah L (12)
|
FGGRSC |
36.47 |
37.15 |
0.68 |
| 8 |
Abril, Sofia V (11)
|
FGGRSC |
37.69 |
37.28 |
-0.41 |
| 9 |
Jorge, Sofia (11)
|
FGMACM |
38.25 |
37.30 |
-0.95 |
| 10 |
Grant, Maria P (12)
|
FGGRSC |
37.75 |
37.46 |
-0.29 |
| 11 |
Virto, Maia (12)
|
FGMWA |
37.20 |
38.89 |
1.69 |
| 12 |
Kingston, Sophia (11)
|
FGGRSC |
40.01 |
39.25 |
-0.76 |
| 13 |
Rhett, Katlyn E (11)
|
FGMWA |
39.52 |
39.64 |
0.12 |
| 14 |
Moreno, Sofia (12)
|
FGGRSC |
39.93 |
40.04 |
0.11 |
| 15 |
Ruiz, Anett (11)
|
FGMACM |
44.63 |
40.20 |
-4.43 |
| 16 |
Macedo, Nicole M (12)
|
FGGRSC |
40.31 |
40.60 |
0.29 |
| 17 |
Martinez, Caitlin (12)
|
FGGRSC |
|
41.67 |
- |
| 18 |
Bicerne, Amelie (11)
|
FGMWA |
42.64 |
43.73 |
1.09 |
| 19 |
Santirzo, Kaylie T (12)
|
FGMACM |
46.62 |
44.07 |
-2.55 |
| 20 |
Ortiz, Natalia V (12)
|
FGMWA |
52.38 |
44.40 |
-7.98 |
| 21 |
Mendoza, Sara M (11)
|
FGUN-0 |
43.10 |
44.71 |
1.61 |
| 22 |
Hampton, Shaniya L (11)
|
FGGRSC |
43.40 |
45.36 |
1.96 |
| 23 |
Fogel, Julia (12)
|
FGGRSC |
48.84 |
46.25 |
-2.59 |
| 24 |
Marin, Daniela V (11)
|
FGGRSC |
51.38 |
46.39 |
-4.99 |
| 25 |
Ortega, Shany C (11)
|
FGPST |
47.96 |
46.43 |
-1.53 |
| 26 |
Snyder, Sophia C (11)
|
FGGRSC |
50.62 |
46.49 |
-4.13 |
| 27 |
Barranco, Christina D (11)
|
FGGRSC |
45.42 |
46.57 |
1.15 |
| 28 |
Regueira, Meghan N (11)
|
FGMACM |
|
50.00 |
- |
| 29 |
Lobosky, Antoinette (12)
|
FGBMSC |
|
53.20 |
- |
|
Valecillos, Valentina (11)
|
FGYSF |
|
DQ |
- |
|
Guerrero, Mia S (12)
|
FGMWA |
37.37 |
NS |
- |
|
Paniagua, Salome (11)
|
FGUN-0 |
44.17 |
NS |
- |
|
Tejeda, Sara (12)
|
FGYSF |
|
NS |
- |