| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hahn, Lily (10)
|
MARAD |
35.04 |
35.69 |
0.65 |
| 2 |
Grimley, Taylor O (10)
|
MARAD |
35.80 |
36.93 |
1.13 |
| 3 |
Sergent, Brooke D (10)
|
MALVA |
37.77 |
39.04 |
1.27 |
| 4 |
Traub, Celia B (9)
|
MAPAAC |
40.36 |
40.15 |
-0.21 |
| 5 |
Neilly, Hannah F (10)
|
MARAD |
42.06 |
41.18 |
-0.88 |
| 6 |
Dahlgren, Brenna (9)
|
MAUN |
42.60 |
42.05 |
-0.55 |
| 7 |
Karnhaukhova, Anastasiya (10)
|
MAUN |
|
43.79 |
- |
| 8 |
Piacenti, Lily (10)
|
MAPFY |
46.94 |
43.87 |
-3.07 |
| 9 |
Ritter, Kassie M (9)
|
MAEAST |
|
48.70 |
- |
| 10 |
Otero-Rehrig, Hazel C (9)
|
MALVA |
49.13 |
49.04 |
-0.09 |
| 11 |
Nagy, Samantha K (9)
|
MAEAST |
50.67 |
49.76 |
-0.91 |
| 12 |
Herman, Alyvia L (9)
|
MAPAAC |
50.67 |
51.88 |
1.21 |
| 13 |
Sandt, Meghan M (9)
|
MAEAST |
55.52 |
52.35 |
-3.17 |
| 14 |
Connell, Samantha R (9)
|
MALVA |
57.65 |
53.36 |
-4.29 |
| 15 |
Gandhi, Reva (10)
|
MAPAAC |
57.13 |
54.23 |
-2.90 |
| 16 |
Chukoskie, Emily P (9)
|
MAPAAC |
51.96 |
1:01.83 |
9.87 |
| 17 |
Barroquiero, Addisen C (9)
|
MAEAST |
|
1:16.20 |
- |
|
Bevenour, Holly E (10)
|
MARAD |
41.10 |
NS |
- |