| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Goldblum, McKaley Q (17)
|
NCBLUE |
23.39 |
24.40 |
1.01 |
| 2 |
Parkes, Callie D (16)
|
NCBLAK |
23.32 |
24.96 |
1.64 |
| 3 |
Murtagh, Liza M (16)
|
NCBLUE |
24.04 |
25.02 |
0.98 |
| 4 |
Teeters, Elizabeth G (15)
|
NCBLUE |
24.54 |
25.57 |
1.03 |
| 5 |
Bishop, Avery M (15)
|
NCBLUE |
26.37 |
26.91 |
0.54 |
| 6 |
Ginsberg, Kinsey A (15)
|
NCBLAK |
26.64 |
27.22 |
0.58 |
| 7 |
Phillips, Ellen T (15)
|
NCBLAK |
27.04 |
27.78 |
0.74 |
| 8 |
Hannum, Ellie G (17)
|
NCBLUE |
26.96 |
28.01 |
1.05 |
| 9 |
Hughes, Bailey L (16)
|
NCBLAK |
28.37 |
28.21 |
-0.16 |
| 10 |
Howard, Maren W (16)
|
NCBLUE |
27.72 |
31.12 |
3.40 |
|
Wells, Mary M (18)
|
NCBLAK |
25.10 |
NS |
- |
|
Fairchild, Abigail V (17)
|
NCBLUE |
27.60 |
NS |
- |
|
Reichow, Alana M (16)
|
NCBLUE |
25.81 |
NS |
- |