| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCarthy, Luke T (12)
|
NCECA |
33.26 |
33.72 |
0.46 |
| 2 |
Camacho, Clement D (11)
|
NCUN |
35.21 |
34.69 |
-0.52 |
| 3 |
Hughes, Jackson C (12)
|
NCWOW |
37.36 |
36.02 |
-1.34 |
| 4 |
Hawthorne, Maxwell S (11)
|
NCWOW |
38.19 |
36.69 |
-1.50 |
| 5 |
Arredondo, Nathan B (11)
|
NCWOW |
39.87 |
39.69 |
-0.18 |
| 6 |
House, William E (11)
|
NCUN |
|
40.11 |
- |
| 7 |
Ive, Jethro T (12)
|
NCWOW |
41.51 |
40.61 |
-0.90 |
| 8 |
Marlow, Tiago V (12)
|
NCECA |
40.08 |
41.17 |
1.09 |
| 9 |
Reiniche, Ryan J (11)
|
NCWOW |
40.35 |
42.40 |
2.05 |
| 10 |
Bollenbacher, Stephen W (11)
|
NCWOW |
43.01 |
42.89 |
-0.12 |
| 11 |
Pesko, Gavin E (11)
|
NCECA |
44.86 |
43.97 |
-0.89 |
| 12 |
Healey, Matthew J (11)
|
NCWOW |
46.75 |
44.97 |
-1.78 |
|
Blaylock, Dakota M (11)
|
NCECA |
|
DQ |
- |
|
Nicol, Iain C (12)
|
NCECA |
1:13.41 |
DQ |
- |