| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Setzer, Sean T (12)
|
NCWOW |
32.42 |
31.67 |
-0.75 |
| 2 |
Stuck, Evan J (12)
|
NCWOW |
31.94 |
33.27 |
1.33 |
| 3 |
Durham, William L (12)
|
NCWOW |
34.66 |
34.52 |
-0.14 |
| 4 |
Hawthorne, Maxwell S (10)
|
NCWOW |
37.81 |
35.59 |
-2.22 |
| 5 |
Post, Cian D (11)
|
NCWOW |
34.82 |
35.76 |
0.94 |
| 6 |
Tillery, Wade H (11)
|
NCWOW |
37.42 |
37.65 |
0.23 |
| 7 |
Fary, Elliott K (11)
|
NCCFAC |
37.53 |
38.37 |
0.84 |
| 8 |
Kanoy, Owen D (12)
|
NCCFAC |
42.27 |
38.61 |
-3.66 |
| 9 |
Witmer, William C (11)
|
NCCFAC |
39.87 |
38.79 |
-1.08 |
| 10 |
Neu, Nathan A (9)
|
NCCFAC |
43.16 |
39.31 |
-3.85 |
| 11 |
Whisnant, Mitchell W (11)
|
NCWOW |
41.56 |
40.40 |
-1.16 |
| 12 |
Reiniche, Ryan J (10)
|
NCWOW |
41.44 |
40.97 |
-0.47 |
| 13 |
Sorensen, Nikolas P (12)
|
NCBAC |
42.61 |
41.32 |
-1.29 |
| 14 |
Butner, Stephen A (9)
|
NCWOW |
40.24 |
41.64 |
1.40 |
| 15 |
Hanson, Jack C (10)
|
NCWOW |
40.97 |
41.75 |
0.78 |
| 16 |
Bollenbacher, Stephen W (10)
|
NCWOW |
42.46 |
42.41 |
-0.05 |
| 17 |
McFarland, Scott C (9)
|
NCWOW |
43.67 |
43.03 |
-0.64 |
| 18 |
Nester, Nathaniel G (12)
|
NCBAC |
|
43.13 |
- |
| 19 |
Mejouev, Maxim (11)
|
NCWOW |
45.01 |
44.58 |
-0.43 |
| 20 |
Pace, Andrew N (11)
|
NCBAC |
44.70 |
44.59 |
-0.11 |
| 21 |
Eder, Ryker P (10)
|
NCCFAC |
|
44.80 |
- |
| 22 |
Wang, Maximilian (11)
|
NCWOW |
49.34 |
45.65 |
-3.69 |
| 23 |
Greene, Jackson C (10)
|
NCCFAC |
52.53 |
48.34 |
-4.19 |
| 24 |
Huggins, Linus A (9)
|
NCCFAC |
|
53.84 |
- |
| 25 |
Harris, Joshua B (9)
|
NCWOW |
52.99 |
54.24 |
1.25 |
| 26 |
Jaynes, Bradley T (12)
|
NCBAC |
58.19 |
54.76 |
-3.43 |
| 27 |
Hickman, Blayze B (10)
|
NCBAC |
|
1:00.64 |
- |
| 28 |
Holmes, Jaxon G (9)
|
NCWOW |
1:16.45 |
1:15.08 |
-1.37 |
| 29 |
Fairchild, Philman J (7)
|
NCWOW |
|
1:26.44 |
- |
|
House, William E (10)
|
NCCFAC |
41.83 |
DQ |
- |
|
Spencer, Slater W (5)
|
NCCFAC |
|
DQ |
- |
|
Dumas, Miller L (12)
|
NCWOW |
41.09 |
NS |
- |
|
Keena, Stephen M (9)
|
NCBAC |
48.92 |
NS |
- |