| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eggers, Kaylee J (12)
|
NCRMY |
2:24.48 |
2:23.43 |
-1.05 |
| 2 |
Lac, Tabitha Y (12)
|
NCWOW |
2:34.89 |
2:30.75 |
-4.14 |
| 3 |
Butler, Emily R (12)
|
NCHCAC |
2:41.36 |
2:32.48 |
-8.88 |
| 4 |
Furbay, Caroline D (11)
|
NCCFAC |
2:34.85 |
2:34.25 |
-0.60 |
| 5 |
Bowen, Madison E (11)
|
NCECA |
2:48.09 |
2:37.27 |
-10.82 |
| 6 |
Beasley, Marion A (12)
|
NCECA |
2:47.82 |
2:37.86 |
-9.96 |
| 7 |
Forbes, Abigail R (11)
|
NCCFAC |
2:39.72 |
2:42.56 |
2.84 |
| 8 |
Klue, Isabella M (11)
|
NCECA |
|
2:43.90 |
- |
| 9 |
Flanagan, Emma R (12)
|
NCSSS |
2:54.97 |
2:44.67 |
-10.30 |
| 10 |
Britton, Jaceil A (11)
|
NCCFAC |
|
2:49.83 |
- |
| 11 |
Hood, Madeline S (12)
|
NCECA |
3:02.81 |
2:51.10 |
-11.71 |
| 12 |
Blackmon, Bryn E (12)
|
NCECA |
2:55.20 |
2:53.56 |
-1.64 |
| 13 |
Fleming, Bryn V (12)
|
NCECA |
2:59.08 |
2:53.89 |
-5.19 |
| 14 |
Eder, Cadence S (12)
|
NCCFAC |
|
2:56.35 |
- |
| 15 |
Brown Aguirre, Samantha I (12)
|
NCECA |
|
3:37.71 |
- |
| 16 |
Lynn, Mabry A (11)
|
NCSSS |
3:40.00 |
3:41.59 |
1.59 |
|
Riley, Kenley A (12)
|
NCECA |
3:22.43 |
DQ |
- |
|
Chapman, Caroline P (12)
|
NCECA |
3:17.82 |
DQ |
- |
|
Flanagan, Madeleine G (11)
|
NCSSS |
3:42.60 |
DQ |
- |
|
Kugatow, Bobbie L (12)
|
NCWOW |
3:40.26 |
DQ |
- |
|
Mixa, Olivia (11)
|
NCSSS |
3:59.99 |
NS |
- |