| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stuck, Kelsey M (12)
|
NCWOW |
30.90 |
30.53 |
-0.37 |
| 2 |
Williams, Lexi M (12)
|
NCWOW |
33.08 |
34.15 |
1.07 |
| 3 |
Steele, Eliana C (12)
|
NCWOW |
37.41 |
34.81 |
-2.60 |
| 4 |
Weigand, Kelsey C (11)
|
NCWOW |
33.73 |
35.09 |
1.36 |
| 5 |
Seward, Emerson M (12)
|
NCWOW |
|
37.26 |
- |
| 6 |
Bongkotmanee, Ida P (12)
|
NCWOW |
39.72 |
37.67 |
-2.05 |
| 7 |
Litton, Maegan Grace K (12)
|
NCWOW |
37.52 |
37.74 |
0.22 |
| 8 |
Reaves, Camren G (12)
|
NCWOW |
37.31 |
37.94 |
0.63 |
| 9 |
Bartlett, Kyla E (11)
|
NCWOW |
38.49 |
38.63 |
0.14 |
| 10 |
Santucci, Skylar (12)
|
NCWOW |
40.62 |
40.60 |
-0.02 |
| 11 |
Volety, Amulya M (11)
|
NCWOW |
41.29 |
40.82 |
-0.47 |
| 12 |
Burke, Marissa N (12)
|
NCWOW |
43.97 |
41.02 |
-2.95 |
| 13 |
Keeler, Samantha B (12)
|
NCWOW |
43.26 |
44.23 |
0.97 |
| 14 |
Sterner, Adalyn G (11)
|
NCWOW |
43.81 |
45.44 |
1.63 |
| 15 |
Van Nostrand, Shea A (11)
|
NCWOW |
51.68 |
47.55 |
-4.13 |
|
Metzinger, Mya Z (12)
|
NCWOW |
33.61 |
NS |
- |