| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zyglocke, Lilly M (12)
|
VAHNVR |
5:53.06 |
5:31.57 |
-21.49 |
| 2 |
Butler, Haley D (12)
|
VAWST |
6:06.54 |
5:33.41 |
-33.13 |
| 3 |
Owen, Renee A (11)
|
PVHACC |
|
5:34.23 |
- |
| 4 |
Strohhacker, Reagan A (11)
|
VAHNVR |
5:19.05 |
5:34.74 |
15.69 |
| 5 |
Furrow, Susannah C (12)
|
VATSU |
5:59.98 |
5:39.40 |
-20.58 |
| 6 |
Cypher, Fiona S (11)
|
PVNCAP |
5:36.20 |
5:39.56 |
3.36 |
| 7 |
Gorgol, Madison E (12)
|
MDMAC |
5:41.22 |
5:42.67 |
1.45 |
| 8 |
Kim, Shane E (12)
|
VAWST |
6:23.22 |
5:44.12 |
-39.10 |
| 9 |
Tanner, Hannah M (12)
|
MDCAA |
6:00.68 |
5:52.38 |
-8.30 |
| 10 |
Hall, Kira M (11)
|
VATSU |
6:43.21 |
6:01.38 |
-41.83 |
| 11 |
Holland, Samantha C (12)
|
VATSU |
6:10.00 |
6:01.64 |
-8.36 |
| 12 |
Fletcher, Hayden C (12)
|
PVNCAP |
6:07.41 |
6:08.40 |
0.99 |
| 13 |
Tomlinson, Sadie R (11)
|
MDFSC |
6:14.18 |
6:08.54 |
-5.64 |
| 14 |
Davis, Lily J (12)
|
AMBRY |
5:30.99 |
6:12.57 |
41.58 |
| 15 |
Shifflett, Jillian M (11)
|
PVNCAP |
6:09.37 |
6:14.47 |
5.10 |
| 16 |
Wang, Jenny J (11)
|
MDMAC |
6:50.13 |
6:38.77 |
-11.36 |
| 17 |
Magill, Emily P (11)
|
VAHNVR |
6:32.66 |
6:40.66 |
8.00 |
| 18 |
Ramnarayan, Alayna R (12)
|
MDJCC |
7:20.03 |
6:51.30 |
-28.73 |
| 19 |
Thompson, Grace E (11)
|
VAWST |
7:38.10 |
7:23.67 |
-14.43 |