| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Plaza, Sofia M (12)
|
NCATOM |
28.48 |
27.91 |
-0.57 |
| 2 |
Oettinger, Claire M (12)
|
NCATOM |
29.63 |
28.48 |
-1.15 |
| 3 |
Haviland, Laura E (12)
|
NCATOM |
29.52 |
29.29 |
-0.23 |
| 4 |
Sellner, Emilie M (12)
|
NCATOM |
30.49 |
29.39 |
-1.10 |
| 5 |
Connor, Lila G (11)
|
NCATOM |
31.84 |
30.78 |
-1.06 |
| 6 |
Sanchez, Aryanna I (11)
|
NCWA |
33.28 |
33.14 |
-0.14 |
| 7 |
Williams, Gracie M (12)
|
NCWA |
|
33.23 |
- |
| 8 |
Patterson, Dorothy B (11)
|
NCATOM |
36.19 |
34.48 |
-1.71 |
| 9 |
Ahdieh, Isabelle M (12)
|
NCATOM |
34.31 |
34.56 |
0.25 |
| 10 |
Lawrence, Amelia S (11)
|
NCWA |
36.37 |
34.68 |
-1.69 |
| 11 |
Omoresemi, Maya-Stella F (11)
|
NCATOM |
35.53 |
35.83 |
0.30 |