| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mottershead, Freya L (8)
|
MAUN |
39.20 |
39.39 |
0.19 |
| 2 |
Tallapragada, Harshitha T (8)
|
MAMSA |
44.25 |
42.25 |
-2.00 |
| 3 |
Doig, Melissa (8)
|
MABRY |
44.70 |
43.52 |
-1.18 |
| 4 |
Fan, Brianna Z (8)
|
MAMSA |
47.01 |
44.25 |
-2.76 |
| 5 |
English, Penelope L (8)
|
MAFCA |
48.38 |
44.47 |
-3.91 |
| 6 |
O'Connor, Bryn (8)
|
MADCAA |
47.85 |
44.53 |
-3.32 |
| 7 |
Oros, Solomiya K (8)
|
MAORCA |
44.80 |
44.84 |
0.04 |
| 8 |
Shaffer, Naomi R (8)
|
MAPCAC |
45.31 |
48.14 |
2.83 |
| 9 |
Heckler, Hayley H (8)
|
MAMSA |
49.89 |
48.76 |
-1.13 |
| 10 |
Lalla, Dhru (8)
|
MABGH |
51.98 |
49.21 |
-2.77 |
| 11 |
DeMartinis, Liliana (8)
|
MASERP |
|
49.35 |
- |
| 12 |
Naraga, Kirsten Claire L (8)
|
MAUMAC |
52.41 |
49.69 |
-2.72 |
| 13 |
Merlo, Elizabeth T (8)
|
MABRY |
|
49.70 |
- |
| 14 |
Trotter, Barcley R (8)
|
MAUMAC |
|
50.12 |
- |
| 15 |
Fan, Catherine (8)
|
MAFCA |
|
51.15 |
- |
| 16 |
Brown, Anelia B (8)
|
MAFCA |
1:04.65 |
51.32 |
-13.33 |
| 17 |
Steckel, Annabelle R (6)
|
MAFCA |
54.29 |
53.16 |
-1.13 |
| 18 |
Zack, Rachel L (8)
|
MAFCA |
54.01 |
55.13 |
1.12 |
| 19 |
Moore, Kathryn J (8)
|
MASERP |
55.30 |
55.47 |
0.17 |
| 20 |
Parks, Mariah (8)
|
MAORCA |
1:11.10 |
55.67 |
-15.43 |
| 21 |
Ali, Hanane A (8)
|
MASERP |
|
58.09 |
- |
| 22 |
Amaev, Kaitlin (8)
|
MAORCA |
58.50 |
58.31 |
-0.19 |
| 23 |
Karpinski, Genevieve F (7)
|
MASERP |
|
1:01.14 |
- |
| 24 |
Lu, Shannen G (6)
|
MAFCA |
|
1:04.41 |
- |
| 25 |
Brown, Annabelle V (8)
|
MAFCA |
|
1:04.47 |
- |
|
Lavelle, Sienna R (8)
|
MAFCA |
|
1:07.35 |
- |
|
Diffily-Kenney, Nora (7)
|
MASERP |
|
DQ |
- |
|
Simpkins, Katherine F (6)
|
MAFCA |
1:03.32 |
DQ |
- |
|
Green, Zoey J (5)
|
MAJRAY |
|
DQ |
- |
|
Whitney, Caroline G (6)
|
MAFCA |
|
NS |
- |
|
Kolessar, Kaitlyn R (8)
|
MASSC |
41.86 |
NS |
- |
|
Shadman, Sydney E (6)
|
MAJRAY |
1:16.17 |
NS |
- |