| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alexander, Sinceraty D (11)
|
MRSPAR |
30.01 |
29.57 |
-0.44 |
| 2 |
Iannetta, Ava L (11)
|
MRSPAR |
33.04 |
31.47 |
-1.57 |
| 3 |
Suteja, Kezia S (11)
|
MRNCAC |
30.88 |
31.73 |
0.85 |
| 4 |
Zhu, Angela (11)
|
MRNCAC |
31.41 |
32.58 |
1.17 |
| 5 |
Weglarz, Juliet R (12)
|
MRSPAR |
32.57 |
32.90 |
0.33 |
| 6 |
Javier, Chrisabella K (11)
|
MRAPEX |
37.26 |
37.62 |
0.36 |
| 7 |
Bauer, Demi J (11)
|
MRSPAR |
38.55 |
38.00 |
-0.55 |
| 8 |
Sharpe, Ashley B (11)
|
MRKBM |
39.49 |
39.43 |
-0.06 |
| 9 |
Zhao, Joanna J (11)
|
MRAPEX |
|
40.18 |
- |
| 10 |
DiMarco, AnnaLynn (11)
|
MRSPAR |
39.09 |
40.95 |
1.86 |
| 11 |
Perez, Miranda M (11)
|
MRKBM |
39.49 |
42.42 |
2.93 |
| 12 |
Ramos, Samantha (12)
|
MRKBM |
44.00 |
45.42 |
1.42 |
| 13 |
Vargas, Jadyn S (11)
|
MRKBM |
48.24 |
51.43 |
3.19 |
| 14 |
Bussey, Genesis S (12)
|
MRAPEX |
|
52.48 |
- |
| 15 |
Royal-Smith, Jaisha (12)
|
MRKBM |
50.84 |
52.97 |
2.13 |
| 16 |
Leonard, Kaicey L (12)
|
MRAPEX |
52.69 |
53.89 |
1.20 |
|
Meyerhoeffer, Stella M (11)
|
MRSPAR |
29.28 |
NS |
- |