| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cattie-Watt, Alexander B (10)
|
MRYFD |
43.29 |
41.18 |
-2.11 |
| 1 |
Katselnik, Elan (10)
|
MRFREE |
45.80 |
42.80 |
-3.00 |
| 2 |
Synyakov, Timofey (10)
|
MRMPNY |
43.92 |
41.84 |
-2.08 |
| 2 |
Youssef, Gabriel (10)
|
MRSIAC |
45.27 |
43.16 |
-2.11 |
| 3 |
Wei, Lejen (10)
|
MRWEST |
44.83 |
43.28 |
-1.55 |
| 3 |
Tsitsiashvili, Matthew (10)
|
MRFREE |
47.61 |
51.29 |
3.68 |
| 4 |
Sciortino, Daniel J (10)
|
MRLIE |
44.40 |
44.37 |
-0.03 |
| 5 |
Peng, Bruce (10)
|
MRLIE |
45.95 |
44.88 |
-1.07 |
| 6 |
Hellemeyer, Shane P (10)
|
MRLIE |
46.37 |
45.06 |
-1.31 |
| 7 |
Picker, Owen M (10)
|
MRWEST |
47.15 |
45.61 |
-1.54 |
| 8 |
Veneziano, James (10)
|
MRSIAC |
46.51 |
46.28 |
-0.23 |
| 9 |
Jia, William X (10)
|
MRMPNY |
47.31 |
46.39 |
-0.92 |
| 10 |
Jasiak-Weinberger, Grayson M (10)
|
MRBKR |
48.50 |
46.65 |
-1.85 |
| 11 |
Jorsling, Jamal C (10)
|
MRBAD |
48.39 |
46.93 |
-1.46 |
| 12 |
Afshar, Nicolas (10)
|
MRLIFE |
47.57 |
47.60 |
0.03 |
| 13 |
Deneen, Aidan (10)
|
MRLIE |
46.23 |
48.06 |
1.83 |
|
Audzeyev, Dennis (10)
|
MRFREE |
43.52 |
NS |
- |