| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wallis, Elliott F (10)
|
MRYFD |
46.59 |
42.67 |
-3.92 |
| 1 |
Litman, Owen (10)
|
MRMAKO |
43.63 |
43.59 |
-0.04 |
| 2 |
Bruno, Justin K (10)
|
MRYFD |
44.25 |
43.61 |
-0.64 |
| 3 |
Kharazishvili, Jason (10)
|
MRCENT |
48.54 |
44.57 |
-3.97 |
| 4 |
Cattie-Watt, Alexander B (9)
|
MRYFD |
46.25 |
45.07 |
-1.18 |
| 5 |
Jasiak-Weinberger, Grayson M (9)
|
MRBKR |
48.28 |
46.14 |
-2.14 |
| 6 |
Soeda, Hiroki (10)
|
MRCENT |
45.94 |
46.30 |
0.36 |
| 7 |
Huang, Oliver Y (10)
|
MRYFD |
48.31 |
46.62 |
-1.69 |
| 8 |
Liu, Max (10)
|
MRCENT |
46.73 |
48.16 |
1.43 |
| 9 |
Cadena Jimenez, Logan J (9)
|
MRNSCC |
52.91 |
48.37 |
-4.54 |
| 10 |
Weisburg, Samuel Q (10)
|
MRAPEX |
51.60 |
49.02 |
-2.58 |
| 11 |
Razo, Soren A (9)
|
MRYFD |
51.28 |
49.69 |
-1.59 |
| 12 |
Hsu, Prajna (10)
|
MRCENT |
53.96 |
52.12 |
-1.84 |
| 13 |
Kim, Lincoln S (9)
|
MRYFD |
1:05.28 |
59.47 |
-5.81 |
| 14 |
Garcia, Damian (10)
|
MRKBM |
1:03.31 |
1:06.03 |
2.72 |