| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fiore, Mariella S (10)
|
MAMUHL |
30.46 |
30.99 |
0.53 |
| 2 |
Kissling, Abigail M (10)
|
MAGMAC |
31.91 |
31.83 |
-0.08 |
| 3 |
Laure, Kendall A (10)
|
MACYA |
32.17 |
32.52 |
0.35 |
| 4 |
Foley, Lily E (10)
|
MAHAC |
36.33 |
35.02 |
-1.31 |
| 5 |
Paterno, Veronica S (10)
|
MAHAC |
34.26 |
35.29 |
1.03 |
| 6 |
Kesterson, Avery B (10)
|
MAHAC |
35.90 |
36.51 |
0.61 |
| 7 |
Virabyan, Angelika J (10)
|
MAHAC |
41.73 |
41.49 |
-0.24 |
| 8 |
Brunner, Emma J (10)
|
MACYA |
42.69 |
41.60 |
-1.09 |
| 9 |
Steinkirchner, Kaitlin P (10)
|
MACYA |
41.91 |
42.13 |
0.22 |
| 10 |
Stefanelli, Isabella L (10)
|
MAUN-0 |
47.86 |
44.00 |
-3.86 |
| 11 |
Reeves, Leah M (10)
|
MAKA |
41.64 |
44.42 |
2.78 |
| 12 |
Bistline, Rebecca R (10)
|
MASHIP |
54.76 |
44.91 |
-9.85 |
| 13 |
Mccauley, Kinsey G (10)
|
MACYA |
48.64 |
48.11 |
-0.53 |
|
Novak, Sascha L (10)
|
MAUN-0 |
|
NS |
- |