| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ryland, Claire N (14)
|
MAHAC |
52.76 |
55.37 |
2.61 |
| 2 |
Rippon, Bella M (14)
|
MAHAC |
56.84 |
58.11 |
1.27 |
| 3 |
Woronko, Angela E (14)
|
MAHAC |
55.98 |
58.32 |
2.34 |
| 4 |
Tafuto, Victoria C (14)
|
MAHAC |
57.43 |
58.53 |
1.10 |
| 5 |
Horner, Elizabeth M (14)
|
MALAC |
1:00.26 |
58.98 |
-1.28 |
| 6 |
Madden, Maya J (13)
|
MAHAC |
57.84 |
59.49 |
1.65 |
| 7 |
Eby, Kendall E (13)
|
MALAC |
1:02.78 |
1:00.22 |
-2.56 |
| 8 |
Sandridge, Angelina N (13)
|
MACYA |
1:00.38 |
1:00.26 |
-0.12 |
| 9 |
Emmerling, Anya M (14)
|
MALAC |
1:03.82 |
1:01.31 |
-2.51 |
| 10 |
Nolt, Jaimie N (14)
|
MALAC |
1:03.85 |
1:02.08 |
-1.77 |
| 11 |
Holmes, Jordan A (13)
|
MALAC |
|
1:02.40 |
- |
| 12 |
Kulasinsky, Katrina E (13)
|
MAHAC |
1:04.89 |
1:02.74 |
-2.15 |
| 13 |
Andrews, Samantha J (13)
|
MAHAC |
1:04.14 |
1:02.77 |
-1.37 |
| 14 |
Cox, Bridget B (13)
|
MAHAC |
1:02.36 |
1:03.87 |
1.51 |
| 15 |
Hocker, Lydia C (13)
|
MALAC |
|
1:04.51 |
- |
| 16 |
Kopec, Marissa J (13)
|
MAHAC |
1:09.53 |
1:05.11 |
-4.42 |
| 17 |
Heberlig, Sarah L (13)
|
MALAC |
1:05.61 |
1:05.36 |
-0.25 |
| 18 |
Poland, Allison (14)
|
MACYA |
1:02.19 |
1:06.29 |
4.10 |