| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mahler, Madeline J (7)
|
MAHAC |
25.88 |
24.11 |
-1.77 |
| 2 |
Kesterson, Avery B (8)
|
MAHAC |
26.83 |
24.78 |
-2.05 |
| 3 |
Rickabaugh, Gemma R (7)
|
MAHAC |
24.92 |
25.02 |
0.10 |
| 4 |
Zeigler, Isabella J (8)
|
MACYA |
26.10 |
25.36 |
-0.74 |
| 5 |
Kowitz, Lucy M (8)
|
MALAC |
27.74 |
25.71 |
-2.03 |
| 6 |
Paterson, Mary C (8)
|
MALAC |
31.42 |
28.46 |
-2.96 |
| 7 |
Chappell, Elena (8)
|
MAHAC |
30.05 |
29.71 |
-0.34 |
| 8 |
Kieffer, Margaret K (7)
|
MALAC |
32.18 |
29.88 |
-2.30 |
| 9 |
Fuertes, Allyssa D (7)
|
MALAC |
29.35 |
30.01 |
0.66 |
| 10 |
Paterno, Veronica S (8)
|
MAHAC |
39.53 |
30.90 |
-8.63 |
| 11 |
Pfautz, Mia R (7)
|
MALAC |
40.16 |
34.36 |
-5.80 |
| 12 |
Lawrence, Leyna M (8)
|
MALAC |
43.47 |
38.53 |
-4.94 |
| 13 |
Bhonde, Jheel P (8)
|
MACYA |
47.03 |
46.82 |
-0.21 |
|
Virabyan, Angelika J (8)
|
MAHAC |
|
DQ |
- |
|
Seydel, Madeline S (8)
|
MACYA |
27.32 |
DQ |
- |
|
George, Olivia J (7)
|
MACYA |
|
DQ |
- |
|
Berkstresser, Hannah K (8)
|
MACYA |
|
DQ |
- |