| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Iskra, AJ (10)
|
LEGO |
41.10 |
40.77 |
-0.33 |
| 2 |
McKnight, Landon (10)
|
OHLCCY |
39.73 |
41.20 |
1.47 |
| 3 |
Harbour, Zachary P (9)
|
LEYYN |
41.80 |
43.13 |
1.33 |
| 4 |
Parkinson, Garrett (9)
|
OHLCCY |
43.59 |
43.60 |
0.01 |
| 5 |
Hassan, Joseph D (9)
|
AMSVY |
41.68 |
43.67 |
1.99 |
| 6 |
Prelerson, Jacob J (9)
|
AMSVY |
|
44.72 |
- |
| 7 |
Cassidy, John (9)
|
OHLCCY |
39.96 |
45.17 |
5.21 |
| 8 |
Arent, Lee B (10)
|
LEYYN |
46.93 |
45.33 |
-1.60 |
| 9 |
Eisentraut, Tyler J (10)
|
LECFYN |
45.35 |
47.26 |
1.91 |
| 10 |
Dechristofaro, Elliot J (10)
|
LEYYN |
46.56 |
47.37 |
0.81 |
| 11 |
Clutter, Chase (10)
|
OHAY |
|
47.55 |
- |
| 12 |
Turnbull, Alexander M (9)
|
LECFYN |
43.87 |
47.61 |
3.74 |
| 13 |
Cramer, August (10)
|
NCY |
44.98 |
49.36 |
4.38 |
| 14 |
Dorenkott, Evan (10)
|
TUSC |
48.27 |
51.63 |
3.36 |
| 15 |
Levkulich, Johnny (9)
|
OHLCWE |
50.56 |
52.75 |
2.19 |
| 16 |
Cunningham, Kaden (10)
|
LEGYB |
51.44 |
54.13 |
2.69 |
| 17 |
Smelko, Ayden P (9)
|
LEGO |
52.04 |
59.90 |
7.86 |
| 18 |
Bodine, Vander M (10)
|
LEYYN |
1:00.70 |
1:03.17 |
2.47 |
|
Stinziano, Joseph (9)
|
OHAY |
47.15 |
NS |
- |
|
Minotas, Braden (9)
|
OHLCWE |
43.22 |
NS |
- |
|
Cullivan, Casey (9)
|
OHLCCY |
49.51 |
NS |
- |