| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Claugus, Katelyn (12)
|
OHLYBD |
31.60 |
30.93 |
-0.67 |
| 2 |
Kiraly, Lillian J (11)
|
LEYYN |
32.05 |
30.98 |
-1.07 |
| 3 |
Miller, Ella B (11)
|
LEYYN |
30.63 |
31.03 |
0.40 |
| 4 |
Minotas, Gianna (12)
|
OHLCWE |
31.09 |
31.18 |
0.09 |
| 5 |
Murray, Alyson (12)
|
LEYYN |
31.73 |
31.36 |
-0.37 |
| 6 |
Munnell, Addi (11)
|
NCCY |
32.09 |
31.98 |
-0.11 |
| 7 |
Swartzwelder, Laina C (12)
|
LEGYB |
31.31 |
32.50 |
1.19 |
| 8 |
Cerone, Abby G (11)
|
LEGYB |
32.52 |
32.81 |
0.29 |
| 9 |
Claugus, Leah (11)
|
OHLYBD |
37.13 |
34.05 |
-3.08 |
| 10 |
Rohr, Sydney (12)
|
OHMLY |
|
34.53 |
- |
| 11 |
Kocher, Kailynn (11)
|
OHLYBD |
36.23 |
34.77 |
-1.46 |
| 12 |
Braccio, Alyssa (12)
|
DYP |
37.36 |
35.96 |
-1.40 |
| 13 |
Satterfield, Sadie E (11)
|
LEYYN |
41.12 |
38.58 |
-2.54 |
| 14 |
Collins, Leah (12)
|
OHLYBD |
36.96 |
39.67 |
2.71 |
| 15 |
Holliday, Rowan (11)
|
NCY |
40.29 |
41.14 |
0.85 |
| 16 |
Reardon, Victoria (12)
|
NCCY |
40.33 |
42.94 |
2.61 |
| 17 |
Calvert, Rylie (11)
|
NCY |
48.83 |
43.43 |
-5.40 |
| 18 |
Martin, Georgia (11)
|
LELKY |
44.40 |
44.63 |
0.23 |
| 19 |
Wilson, Emma D (11)
|
LEVFYT |
42.58 |
46.00 |
3.42 |
| 20 |
Seesan, Isabelle (11)
|
LELKY |
|
48.31 |
- |
| 21 |
Gawry, Abby E (11)
|
LEVFYT |
58.92 |
56.27 |
-2.65 |
|
Marrero, Jesmari (12)
|
LEVFYT |
42.42 |
NS |
- |