| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guess, Leah (13)
|
NCY |
28.93 |
28.37 |
-0.56 |
| 2 |
Peterson, Sydney M (13)
|
LEGYB |
28.54 |
28.85 |
0.31 |
| 3 |
Gemmen, Ruthie (13)
|
OHLCWE |
28.77 |
28.99 |
0.22 |
| 4 |
Scheibelhoffer, Olivia G (13)
|
LEGYB |
28.47 |
29.18 |
0.71 |
| 5 |
Rockenberger, Elizabeth A (14)
|
LEGO |
29.09 |
29.35 |
0.26 |
| 6 |
Berrow, Annie (13)
|
OHLCCY |
34.12 |
29.55 |
-4.57 |
| 7 |
Perticarini, Anna (14)
|
NCY |
29.47 |
30.42 |
0.95 |
| 8 |
Meyer, Avery (13)
|
LELKY |
|
31.22 |
- |
| 9 |
Murray, Samantha (13)
|
LEYYN |
32.75 |
34.08 |
1.33 |
| 10 |
Embry, Ella G (13)
|
LECFYN |
35.02 |
34.84 |
-0.18 |
| 11 |
Dorfi, Grace (14)
|
AMSVY |
36.88 |
37.28 |
0.40 |
| 12 |
Vlaiku, Cadence E (14)
|
LEYYN |
39.57 |
38.39 |
-1.18 |
| 13 |
Valdez, Emilia I (14)
|
LECFYN |
42.31 |
43.46 |
1.15 |
|
Farcas, Gabrielle (13)
|
LELKY |
|
NS |
- |
|
Gibson, Rachel (13)
|
LELKY |
|
NS |
- |
|
Meyer, Sydney (13)
|
LELKY |
|
NS |
- |