| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ham, Annika (12)
|
DYP |
35.48 |
34.29 |
-1.19 |
| 2 |
Crnjak, Petra (12)
|
LEYYN |
35.94 |
35.87 |
-0.07 |
| 3 |
Kiraly, Lillian J (11)
|
LEYYN |
37.50 |
36.08 |
-1.42 |
| 4 |
Claugus, Leah (11)
|
OHLYBD |
37.13 |
37.62 |
0.49 |
| 5 |
Cassidy, Anna (11)
|
OHLCCY |
39.28 |
38.19 |
-1.09 |
| 6 |
Bell, Madelyn G (11)
|
LEYYN |
37.82 |
38.99 |
1.17 |
| 7 |
Munnell, Mimi (11)
|
NCCY |
39.06 |
39.68 |
0.62 |
| 8 |
Satterfield, Sadie E (11)
|
LEYYN |
39.64 |
39.85 |
0.21 |
| 9 |
Flickinger, Rylee (12)
|
NCY |
38.69 |
39.99 |
1.30 |
| 10 |
Carter, Rebecca M (11)
|
LECFYN |
42.28 |
41.03 |
-1.25 |
| 11 |
Cerone, Abby G (11)
|
LEGYB |
42.82 |
42.28 |
-0.54 |
| 12 |
Rohr, Sydney (12)
|
OHMLY |
|
43.81 |
- |
| 13 |
Swartzwelder, Laina C (12)
|
LEGYB |
45.14 |
44.15 |
-0.99 |
| 14 |
Johnston, Addison (12)
|
OHLCCY |
44.28 |
44.89 |
0.61 |
| 15 |
Calvert, Rylie (11)
|
NCY |
45.77 |
45.61 |
-0.16 |
| 16 |
Gawry, Abby E (11)
|
LEVFYT |
54.85 |
48.56 |
-6.29 |
| 17 |
Seesan, Isabelle (11)
|
LELKY |
47.90 |
48.77 |
0.87 |
| 18 |
Braccio, Alyssa (12)
|
DYP |
50.85 |
49.17 |
-1.68 |
| 19 |
Melone, Mary G (11)
|
LEYYN |
58.66 |
49.67 |
-8.99 |
| 20 |
Reardon, Victoria (12)
|
NCCY |
48.07 |
50.11 |
2.04 |
| 21 |
Smith, Sabrina R (11)
|
LEGO |
50.07 |
51.56 |
1.49 |
| 22 |
Kelly, Norin A (11)
|
LEYYN |
56.24 |
55.47 |
-0.77 |
| 23 |
Michaels, Emily (11)
|
AMSVY |
59.81 |
1:00.11 |
0.30 |
|
Mastren, Alaina (11)
|
NCCY |
38.77 |
NS |
- |
|
Wickham, Layla (11)
|
LEMBS |
1:05.00 |
NS |
- |