| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kiraly, Lillian J (11)
|
LEYYN |
31.77 |
32.15 |
0.38 |
| 2 |
Hawk, Emma (12)
|
TUSC |
32.69 |
32.37 |
-0.32 |
| 3 |
Ham, Annika (12)
|
DYP |
31.63 |
32.39 |
0.76 |
| 4 |
Murray, Alyson (12)
|
LEYYN |
33.79 |
32.91 |
-0.88 |
| 5 |
Munnell, Addi (11)
|
NCCY |
33.49 |
33.32 |
-0.17 |
| 6 |
Cerone, Abby G (11)
|
LEGYB |
36.39 |
33.47 |
-2.92 |
| 7 |
Rosenbaum, Anna E (12)
|
LEGO |
32.88 |
34.11 |
1.23 |
| 8 |
Leonard, Julia (12)
|
NCY |
36.03 |
34.17 |
-1.86 |
| 9 |
Claugus, Leah (11)
|
OHLYBD |
34.95 |
34.51 |
-0.44 |
| 10 |
Kocher, Kailynn (11)
|
OHLYBD |
35.44 |
35.10 |
-0.34 |
| 11 |
Hanek, Lauren S (11)
|
LEGO |
35.15 |
35.79 |
0.64 |
| 12 |
Salmon, Katie A (11)
|
LEGYB |
35.60 |
36.43 |
0.83 |
| 13 |
Schneider, Katherine L (12)
|
LEGO |
37.97 |
37.19 |
-0.78 |
| 14 |
Cassidy, Anna (11)
|
OHLCCY |
37.04 |
37.63 |
0.59 |
| 15 |
Harbour, Sydney (12)
|
NCY |
34.02 |
37.69 |
3.67 |
| 16 |
Marino, Gianna (11)
|
LEYYN |
37.96 |
38.56 |
0.60 |
| 17 |
Ascani, Gabriella (11)
|
NCY |
36.97 |
38.73 |
1.76 |
| 18 |
Johnston, Addison (12)
|
OHLCCY |
40.54 |
40.21 |
-0.33 |
| 19 |
Calvert, Rylie (11)
|
NCY |
39.07 |
41.22 |
2.15 |
| 20 |
Reardon, Victoria (12)
|
NCCY |
39.09 |
42.84 |
3.75 |
| 21 |
Burbank-Williams, Destinee (11)
|
LEMBS |
40.98 |
43.05 |
2.07 |
| 22 |
Carter, Rebecca M (11)
|
LECFYN |
42.77 |
43.74 |
0.97 |
| 23 |
Licata, Isabella D (11)
|
LEYYN |
43.12 |
43.79 |
0.67 |
| 24 |
Prelerson, Jordan (11)
|
AMSVY |
44.85 |
44.82 |
-0.03 |
| 25 |
Martin, Georgia (11)
|
LELKY |
44.85 |
45.07 |
0.22 |
| 26 |
Wickham, Layla (11)
|
LEMBS |
47.70 |
47.92 |
0.22 |
| 27 |
Kelly, Norin A (11)
|
LEYYN |
45.06 |
48.70 |
3.64 |
|
Michaels, Emily (11)
|
AMSVY |
42.26 |
NS |
- |
|
Marrero, Jesmari (12)
|
LEVFYT |
42.08 |
NS |
- |
|
Miller, Ella B (11)
|
LEYYN |
36.53 |
NS |
- |