| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitaker, Brock H (10)
|
MASSC |
33.92 |
33.32 |
-0.60 |
| 2 |
D'Ariano, Landon N (10)
|
MASSC |
34.65 |
33.54 |
-1.11 |
| 3 |
Zagaceta, Magill M (9)
|
MAHAC |
34.93 |
34.17 |
-0.76 |
| 4 |
Potts, Mason W (10)
|
MANPAC |
34.84 |
34.34 |
-0.50 |
| 5 |
Moyer, John D (10)
|
MAJW |
35.12 |
34.95 |
-0.17 |
| 6 |
Woren, Joshua L (10)
|
MACVAC |
36.42 |
35.37 |
-1.05 |
| 7 |
Almodovar, Jayden J (10)
|
MAMARA |
35.09 |
35.93 |
0.84 |
| 8 |
Chang, Jonathan H (10)
|
MAWSY |
36.16 |
36.08 |
-0.08 |
| 9 |
Marks, Carter R (10)
|
MALCY |
37.12 |
36.36 |
-0.76 |
| 10 |
Fleck, Brandon G (9)
|
MATHY |
38.24 |
36.90 |
-1.34 |
| 11 |
Estep, Derek T (9)
|
MASHIP |
39.09 |
37.01 |
-2.08 |
| 12 |
Zhou, Andrew R (10)
|
MAPWAC |
37.26 |
37.25 |
-0.01 |
| 13 |
Chen, Conan C (9)
|
MARAD |
38.17 |
37.64 |
-0.53 |
| 14 |
Mortellite, Anthony M (10)
|
MAUN-0 |
38.26 |
37.69 |
-0.57 |
| 15 |
Chiu, Brandon (9)
|
MAFSS |
38.98 |
37.72 |
-1.26 |
| 16 |
Wang, Jake (10)
|
MASSC |
36.34 |
38.01 |
1.67 |
| 17 |
Rees, Jonah A (10)
|
MAYY |
38.21 |
38.09 |
-0.12 |
| 18 |
Pervall, Ian C (10)
|
MADCAA |
37.14 |
38.17 |
1.03 |
| 18 |
Saeger, Thomas (10)
|
MAUPY |
37.70 |
38.17 |
0.47 |
| 20 |
Tsaturyan, Alec (10)
|
MAPWAC |
38.19 |
38.33 |
0.14 |
| 21 |
Armstrong, Patrick K (10)
|
MAPVA |
33.81 |
39.12 |
5.31 |
| 22 |
Lemay, Zachery E (10)
|
MACBFY |
38.67 |
39.30 |
0.63 |
| 23 |
Hang, Kai V (10)
|
MAUPY |
38.24 |
39.88 |
1.64 |
| 24 |
Kalish, Carson R (9)
|
MADST |
38.86 |
40.26 |
1.40 |
| 25 |
Haney, James (10)
|
MAPVA |
37.44 |
40.39 |
2.95 |
| 26 |
Schnapf, Aidan M (10)
|
MACBST |
38.94 |
40.79 |
1.85 |