| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Madelaine O (17)
|
NCSTAR |
23.73 |
23.70 |
-0.03 |
| 2 |
Fulcher, Courtney C (17)
|
NCWAVE |
24.49 |
24.75 |
0.26 |
| 3 |
Hall, Sydney V (17)
|
VAODAC |
25.38 |
25.20 |
-0.18 |
| 4 |
Heineman, Ashley M (17)
|
NCWAVE |
24.79 |
25.59 |
0.80 |
| 5 |
Harper, Jasmine F (17)
|
VANOVA |
25.63 |
25.73 |
0.10 |
| 6 |
Bowers, Anne C (18)
|
NCASC |
25.52 |
26.02 |
0.50 |
| 7 |
Jewell, Aleta M (18)
|
NCWOW |
26.25 |
26.34 |
0.09 |
| 8 |
Lai, Allison (17)
|
NCCAT |
25.29 |
26.59 |
1.30 |
| 9 |
Melton, Alexandra M (18)
|
VANOVA |
26.27 |
26.79 |
0.52 |
| 10 |
Ueland, Hannah E (17)
|
NCRSA |
27.02 |
26.80 |
-0.22 |
| 11 |
Howard, Emma B (17)
|
NCCAT |
25.80 |
26.92 |
1.12 |
| 12 |
Montgomery, Shelby G (17)
|
KYGSCS |
26.52 |
27.01 |
0.49 |
| 13 |
Bryant, Sophia A (18)
|
NCSAIL |
26.45 |
27.22 |
0.77 |
| 14 |
Kerr, Lily F (17)
|
SCH2A |
26.54 |
27.51 |
0.97 |
| 15 |
Milem, Emma G (17)
|
NCRACY |
27.17 |
27.53 |
0.36 |
| 16 |
Volz, Julia R (17)
|
NCSAIL |
27.25 |
28.07 |
0.82 |
| 17 |
MacKinnon, Anna H (17)
|
NCCAT |
27.86 |
28.09 |
0.23 |
| 18 |
Traugot, Laura R (18)
|
NCRSA |
27.49 |
28.24 |
0.75 |
| 19 |
Michie, Kayleigh M (17)
|
MASTRM |
28.24 |
28.30 |
0.06 |
| 20 |
Jorgensen, Sarah R (17)
|
VANOVA |
28.98 |
28.53 |
-0.45 |
| 21 |
Shaughnessy, Rachael M (17)
|
NCCAT |
27.20 |
28.81 |
1.61 |
| 22 |
Reeves, Cassidy J (17)
|
NCWAVE |
29.42 |
28.97 |
-0.45 |
| 23 |
Little, Ashley M (17)
|
NCRSA |
28.66 |
29.45 |
0.79 |
| 24 |
Hayward, Alyssa L (17)
|
SCH2A |
29.15 |
29.85 |
0.70 |
| 25 |
Fields, Katye M (17)
|
NCSTAR |
30.52 |
30.04 |
-0.48 |
| 26 |
Allen, Kathryn R (17)
|
SCH2A |
28.74 |
30.17 |
1.43 |
|
Van Tassel, Lindsey S (17)
|
NCRSA |
26.34 |
NS |
- |
|
Hon, Gwyneth G (17)
|
NCBAC |
35.08 |
NS |
- |
|
Payne, Victoria N (17)
|
NCASC |
28.78 |
NS |
- |