| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thomas, Tia (13)
|
PVRMSC |
24.34 |
24.45 |
0.11 |
| 2 |
Cash, Joanie (13)
|
MRLIAC |
24.56 |
24.87 |
0.31 |
| 3 |
Bush, Malia (13)
|
PVRMSC |
25.05 |
25.33 |
0.28 |
| 4 |
Erickson, Olivia S (13)
|
VANOVA |
25.36 |
25.37 |
0.01 |
| 5 |
Runnels, Aris D (13)
|
PVNCAP |
25.59 |
25.38 |
-0.21 |
| 6 |
Wallace, Taylor O (13)
|
PVRMSC |
25.31 |
25.52 |
0.21 |
| 7 |
Skopp-Cardillo, Juliana C (13)
|
PVNCAP |
25.90 |
25.73 |
-0.17 |
| 8 |
Thomas, Nianna R (13)
|
MRLIAC |
26.10 |
26.09 |
-0.01 |
| 9 |
Ahmed, Ellie M (13)
|
VANOVA |
25.62 |
26.44 |
0.82 |
| 10 |
Weaverling, Catherine A (13)
|
MADST |
25.77 |
26.89 |
1.12 |
| 11 |
Caruccio, Amanda J (13)
|
MADST |
26.81 |
27.39 |
0.58 |
| 12 |
Kamor, Adriana T (13)
|
MRLIAC |
28.61 |
28.42 |
-0.19 |
| 13 |
Pittala, Sreeya (13)
|
MADST |
33.30 |
29.14 |
-4.16 |
|
Ackerly, Margaret W (13)
|
VAUN |
25.64 |
25.44 |
-0.20 |
|
O'Hagan, Ciara E (13)
|
MRLIAC |
28.81 |
28.49 |
-0.32 |
|
Stovall, Zora L (13)
|
PVNCAP |
26.25 |
NS |
- |