| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Cheyenne F (12)
|
PVRMSC |
26.01 |
25.82 |
-0.19 |
| 2 |
Anderson, Sarah M (12)
|
PVNCAP |
26.72 |
26.19 |
-0.53 |
| 3 |
Schneider, Alexandra G (12)
|
MRLIAC |
26.56 |
26.33 |
-0.23 |
| 4 |
Cunnane, Mackenzie I (12)
|
PVNCAP |
26.57 |
26.35 |
-0.22 |
| 5 |
Makin, Emily M (12)
|
PVNCAP |
26.17 |
26.42 |
0.25 |
| 6 |
Peterson, Sanna B (12)
|
VANOVA |
26.41 |
26.59 |
0.18 |
| 7 |
DeBaecke, Sydney E (12)
|
MADST |
27.06 |
27.10 |
0.04 |
| 8 |
Zoltick, Stephanie R (12)
|
PVRMSC |
27.13 |
27.16 |
0.03 |
| 9 |
Bowrin, Nia A (12)
|
PVRMSC |
27.17 |
27.49 |
0.32 |
| 10 |
Haag, Brooke N (12)
|
MRLIAC |
28.12 |
27.78 |
-0.34 |
| 11 |
Wright, Alex M (12)
|
VANOVA |
27.46 |
28.15 |
0.69 |
| 12 |
Anderson, Emma R (12)
|
MRLIAC |
29.07 |
28.65 |
-0.42 |
|
Macholz, Amelia B (12)
|
VAUN |
26.99 |
26.92 |
-0.07 |
|
Leng, Claire (12)
|
MRLIAC |
29.16 |
27.89 |
-1.27 |