| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Camurdan, Atesh B (13)
|
VANOVA |
50.30 |
51.41 |
1.11 |
| 2 |
Fitch, David R (13)
|
PVNCAP |
52.70 |
51.64 |
-1.06 |
| 3 |
Rooney, Aiden P (13)
|
PVRMSC |
53.28 |
52.83 |
-0.45 |
| 4 |
Tran, Andrew H (13)
|
PVNCAP |
52.84 |
53.15 |
0.31 |
| 5 |
Dietrich, Chandler J (13)
|
PVNCAP |
53.64 |
53.85 |
0.21 |
| 6 |
Shopis, Nicholas (13)
|
MRLIAC |
54.17 |
54.10 |
-0.07 |
| 7 |
Mitchell, Cole (13)
|
PVRMSC |
53.85 |
54.18 |
0.33 |
| 8 |
Nachod, Benjamin E (13)
|
PVRMSC |
54.18 |
55.58 |
1.40 |
| 9 |
Mistretta, Dominic A (13)
|
VANOVA |
57.87 |
56.83 |
-1.04 |
| 10 |
Shopis, Eric C (13)
|
MRLIAC |
1:00.51 |
57.03 |
-3.48 |
| 11 |
Yan, Patrick (13)
|
MRLIAC |
59.53 |
57.96 |
-1.57 |
| 12 |
Jobe, Dalton E (13)
|
VANOVA |
|
58.27 |
- |
| 13 |
Zakielarz, William M (13)
|
MADST |
58.93 |
58.71 |
-0.22 |
|
Hoch, Andrew R (13)
|
MADST |
58.07 |
NS |
- |
|
Wilmore, Aaron L (13)
|
MADST |
56.79 |
NS |
- |