| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dillman, Shannah R (12)
|
VANOVA |
30.64 |
30.26 |
-0.38 |
| 2 |
Rogerson, Anneliese N (12)
|
VANOVA |
32.52 |
32.91 |
0.39 |
| 3 |
Tingen, Casey L (11)
|
VAWAC |
32.49 |
33.09 |
0.60 |
| 4 |
Deane, Kacey E (11)
|
VATAC |
32.52 |
33.51 |
0.99 |
| 5 |
Weir, Catherine A (12)
|
VANOVA |
37.64 |
34.53 |
-3.11 |
| 6 |
Anderson, Chloe E (12)
|
VAWAC |
33.74 |
34.70 |
0.96 |
| 7 |
Sushkoff, Alexandra R (12)
|
VANOVA |
34.27 |
35.01 |
0.74 |
| 8 |
Hanks, Lindsay N (11)
|
VAWAC |
33.91 |
35.04 |
1.13 |
| 9 |
Vincent, Claudia R (11)
|
VANOVA |
34.02 |
35.71 |
1.69 |
| 10 |
Morris, Peyton C (12)
|
VANOVA |
45.50 |
35.90 |
-9.60 |
| 11 |
DeRosier, Olivia S (12)
|
VANOVA |
35.02 |
36.12 |
1.10 |
| 12 |
West, Lillian E (12)
|
VANOVA |
35.35 |
36.33 |
0.98 |
|
McMahan, Ainsley G (11)
|
VAWAC |
|
DQ |
- |
|
Armstrong, Anna S (12)
|
VANOVA |
34.61 |
NS |
- |
|
Brumley, Campbell G (12)
|
VANOVA |
33.93 |
NS |
- |