| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crouthamel, Lauren C (7)
|
MAWWC |
50.22 |
48.68 |
-1.54 |
| 2 |
Doppler, Molly E (7)
|
MAPRSC |
49.53 |
49.87 |
0.34 |
| 3 |
Nordberg, Kaitlyn G (7)
|
MAWWC |
48.98 |
51.35 |
2.37 |
| 4 |
McGlinchey, Simone I (7)
|
MAGPAC |
53.38 |
51.51 |
-1.87 |
| 5 |
Kelsey, Madison R (7)
|
MAECCC |
49.76 |
51.69 |
1.93 |
| 6 |
Petrillo, Jenna A (7)
|
MATWAC |
50.83 |
52.02 |
1.19 |
| 7 |
Geberth, Rebecca L (7)
|
MATHY |
55.87 |
52.64 |
-3.23 |
| 8 |
Briscoe, Chanelle N (7)
|
MAGPAC |
54.72 |
55.06 |
0.34 |
| 9 |
Lesovitz, Neely A (7)
|
MAFSS |
|
56.56 |
- |
| 10 |
DeVito, Isabella C (7)
|
MAGPAC |
|
57.82 |
- |
| 11 |
Carroll, Teagan R (7)
|
MASTRM |
55.00 |
58.21 |
3.21 |
| 12 |
Schroeder, Caroline K (7)
|
MAECCC |
1:03.26 |
59.36 |
-3.90 |
| 13 |
Hamnett, Emily A (7)
|
MATHY |
1:07.02 |
59.55 |
-7.47 |
| 14 |
Colon, Karrington R (7)
|
MAGPAC |
|
59.96 |
- |
| 15 |
Mantini, Elena B (7)
|
MAFSS |
|
1:02.53 |
- |
| 16 |
Shadman, Sydney E (7)
|
MASTRM |
|
1:05.56 |
- |
| 17 |
Knoflicek, Winifred E (7)
|
MAFSS |
|
1:09.35 |
- |
|
Blizzard, Mackenzie E (7)
|
MAECCC |
48.98 |
NS |
- |