| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleck, Brandon G (10)
|
MATHY |
28.34 |
27.87 |
-0.47 |
| 2 |
Levine, Aidan (10)
|
MRTRI |
27.93 |
28.21 |
0.28 |
| 3 |
Rees, Davis J (9)
|
MAMAC |
29.08 |
28.89 |
-0.19 |
| 4 |
Gutin, David E (10)
|
MRMPNY |
29.95 |
29.42 |
-0.53 |
| 5 |
Vradinskiy, Sebastian (10)
|
MATHY |
29.30 |
29.51 |
0.21 |
| 6 |
Smilevski, Maximilian (10)
|
MASEA |
30.58 |
29.55 |
-1.03 |
| 6 |
Weise, Braden E (10)
|
MAGMAC |
29.37 |
29.55 |
0.18 |
| 8 |
Cummings, Cornelius E (10)
|
MAPACS |
30.94 |
29.82 |
-1.12 |
| 9 |
Jenza, Nikita (9)
|
MATHY |
30.32 |
30.14 |
-0.18 |
| 10 |
Dunn, Liam (10)
|
MRTRI |
32.02 |
30.40 |
-1.62 |
| 11 |
Miao, Owen (10)
|
MRLGAC |
30.54 |
30.51 |
-0.03 |
| 12 |
Taylor, Ryan A (10)
|
MALAC |
30.32 |
30.61 |
0.29 |
| 13 |
Veit, Colin (10)
|
MRTVSC |
30.17 |
30.66 |
0.49 |
| 14 |
Chan, Beau (10)
|
MRMAKO |
30.20 |
30.75 |
0.55 |
| 15 |
Bazylko, Gregory (10)
|
MRMPNY |
30.25 |
30.76 |
0.51 |
| 16 |
McDonough, Lucas G (10)
|
MALMOR |
30.47 |
30.90 |
0.43 |
| 17 |
Stevens, Coleman D (10)
|
MAFSSC |
30.13 |
30.99 |
0.86 |
| 18 |
Tran, Gavin H (9)
|
MAHAC |
31.18 |
31.19 |
0.01 |
| 19 |
Sommerstad, Ryan T (10)
|
MRTVSC |
30.93 |
31.59 |
0.66 |
| 20 |
Falk, Brian J (10)
|
MASEA |
35.32 |
32.25 |
-3.07 |
| 20 |
Savov, Alex (10)
|
MASEA |
31.78 |
32.25 |
0.47 |