| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Columbia, Maci G (10)
|
NCFAST |
41.98 |
41.00 |
-0.98 |
| 2 |
Claborn, Alyssa A (10)
|
NCLOY |
43.46 |
41.86 |
-1.60 |
| 3 |
Bellissimo, Sophia G (10)
|
NCLOY |
44.16 |
42.01 |
-2.15 |
| 4 |
Bolyard, Macy J (10)
|
NCGCY |
|
44.10 |
- |
| 5 |
McDonough, Addyson R (10)
|
NCLOY |
49.34 |
45.14 |
-4.20 |
| 6 |
Nannucci, Bianca (9)
|
NCGCY |
53.56 |
46.20 |
-7.36 |
| 7 |
Wehe, Naomi L (10)
|
NCGCY |
|
46.31 |
- |
| 8 |
Misener, Leah J (9)
|
NCLOY |
46.92 |
46.72 |
-0.20 |
| 9 |
Filiault, Julia K (10)
|
NCLOY |
53.86 |
47.89 |
-5.97 |
| 10 |
Okoniewski, Kayla M (10)
|
NCLOY |
|
49.51 |
- |
| 13 |
Johnson, Emory R (9)
|
NCFAST |
58.01 |
54.97 |
-3.04 |
| 16 |
Dingman, Kasey S (9)
|
NCGCY |
|
57.67 |
- |
| 18 |
Bird, Alexa E (10)
|
NCGCY |
59.45 |
59.64 |
0.19 |
| 19 |
Grubb, Emma M (9)
|
NCGCY |
1:03.40 |
1:03.49 |
0.09 |
|
Hua, Claire S (10)
|
NCGCY |
53.34 |
NS |
- |
|
Kornatz, Maeve D (9)
|
NCGCY |
|
NS |
- |