| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Katzer, Danika J (16)
|
VACGBD |
2:38.76 |
2:48.25 |
9.49 |
| 2 |
Salgado, Lauren I (16)
|
VAODAC |
2:48.53 |
2:55.53 |
7.00 |
| 3 |
Berry, Milana G (18)
|
VATIDE |
2:48.94 |
2:56.96 |
8.02 |
| 4 |
Tinneny, Jacqueline M (16)
|
VACGBD |
2:48.22 |
2:57.15 |
8.93 |
| 5 |
Smith, Julia A (15)
|
VATIDE |
2:59.68 |
3:00.59 |
0.91 |
| 6 |
Miller, Morgan A (16)
|
VAUCGB |
3:03.56 |
3:01.19 |
-2.37 |
| 7 |
Watson, Shelby C (16)
|
VA757 |
2:50.33 |
3:05.42 |
15.09 |
| 8 |
Pegram, Kaitlyn A (15)
|
VA757 |
3:16.53 |
3:15.47 |
-1.06 |
| 9 |
Oliver, Julia S (16)
|
VAECAT |
3:06.38 |
3:18.57 |
12.19 |
| 10 |
Geiszler, Peyton K (15)
|
VANOVA |
3:14.87 |
3:19.21 |
4.34 |
| 11 |
Knepper, Celeste M (17)
|
VADIG |
3:12.60 |
3:27.93 |
15.33 |
| 12 |
Hall, Sydney V (17)
|
VAODAC |
3:26.35 |
3:28.92 |
2.57 |
| 13 |
Neikirk, Alexandra L (15)
|
VACGBD |
3:17.72 |
3:29.18 |
11.46 |
| 14 |
Abraham, Marynna G (16)
|
VAECAT |
3:28.88 |
3:31.51 |
2.63 |
| 15 |
Triolet, Isabella R (15)
|
VAODAC |
3:43.97 |
3:38.15 |
-5.82 |