| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hurleman, Hannah T (14)
|
MALAC |
52.85 |
54.16 |
1.31 |
| 2 |
Friedlander, Ellie S (14)
|
MALACU |
55.85 |
58.18 |
2.33 |
| 3 |
Fickett, Makennah J (14)
|
MAHAC |
53.89 |
59.01 |
5.12 |
| 4 |
Parry, Kayla G (13)
|
MALAC |
1:01.91 |
59.59 |
-2.32 |
| 5 |
Dye, Amelia E (14)
|
MAHAC |
1:00.01 |
1:00.65 |
0.64 |
| 6 |
Bookamyer, Caitlyn E (13)
|
MACYA |
1:01.16 |
1:02.33 |
1.17 |
| 7 |
Epoca, Kelsey L (14)
|
MAHAC |
1:03.44 |
1:03.25 |
-0.19 |
| 8 |
Tate, Juliana R (13)
|
MACYA |
1:05.25 |
1:06.34 |
1.09 |
| 9 |
Repkoe, Catherine M (13)
|
MALAC |
1:06.99 |
1:06.87 |
-0.12 |
| 10 |
Anderson, Kari N (13)
|
MALAC |
1:08.11 |
1:10.92 |
2.81 |