| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weston, Lily A (12)
|
MAFSA |
|
42.96 |
- |
| 2 |
Seidman, Jordan K (11)
|
MAGAAC |
43.76 |
43.31 |
-0.45 |
| 3 |
Rasmussen, Rebecca S (12)
|
MAFSA |
45.38 |
43.36 |
-2.02 |
| 4 |
Brasch, Hannah L (12)
|
NJLIFE |
47.67 |
43.52 |
-4.15 |
| 5 |
Doran, Erika L (11)
|
MAUNAT |
44.80 |
44.48 |
-0.32 |
| 6 |
Freeman, Sarah E (11)
|
MAGAAC |
43.46 |
45.77 |
2.31 |
| 7 |
Cosgrove, Scarlett J (11)
|
MAGAAC |
44.39 |
46.31 |
1.92 |
| 8 |
Ortiz, Astashi (12)
|
NJLIFE |
55.78 |
52.94 |
-2.84 |
| 9 |
Nartey, Afia S (11)
|
MASERP |
1:00.27 |
56.80 |
-3.47 |
|
Mejia-Ranno, Nicolette I (12)
|
MAJRAY |
57.08 |
NS |
- |