| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnston, Sabrina K (13)
|
MRBGNW |
|
52.09 |
- |
| 2 |
Normark, Elizabeth C (17)
|
GAMAAC |
|
52.85 |
- |
| 3 |
Carper, Ashley N (16)
|
MAGPAC |
|
53.06 |
- |
| 4 |
Payne, Grayson E (16)
|
SEMCC |
|
53.68 |
- |
| 5 |
Misenar, Morgan A (16)
|
SEBSC |
|
53.74 |
- |
| 6 |
Wilson, Hallie M (16)
|
VAPSDN |
|
53.80 |
- |
| 7 |
McCurdy, Caroline G (13)
|
NEBGSC |
|
53.83 |
- |
| 8 |
Miller, Ella M (16)
|
NEEDGE |
|
53.92 |
- |
| 9 |
Higgins, Sydney V (17)
|
MIEGRA |
|
53.94 |
- |
| 10 |
Eaton, Julianna I (17)
|
PCMP |
|
54.00 |
- |
| 11 |
Lee, Lauren E (16)
|
ILFOX |
|
54.15 |
- |
| 12 |
Levine, Laura J (16)
|
MIEGRA |
|
54.84 |
- |
| 13 |
Engesser, Julianna A (16)
|
ILSCST |
|
54.96 |
- |
| 14 |
Balow, Sophia K (16)
|
MIPCC |
|
55.39 |
- |
| 15 |
Israels, Emma K (15)
|
MIEGRA |
|
55.79 |
- |
| 16 |
Reicherter, Mary Kate (15)
|
PVAAC |
|
56.55 |
- |
|
Clark, Jessica M (16)
|
MIPCC |
|
DQ |
- |