| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Connelly, Lauren V (12)
|
PVNCAP |
34.08 |
38.67 |
4.59 |
| 2 |
Dugan, Naomi E (12)
|
PVNCAP |
34.02 |
38.74 |
4.72 |
| 3 |
Rand, Zoe C (12)
|
PVNCAP |
34.77 |
39.17 |
4.40 |
| 4 |
Wilhite, Charlotte L (11)
|
PVMACH |
36.53 |
40.67 |
4.14 |
| 5 |
Bryan, Elizabeth M (9)
|
PVMACH |
36.24 |
42.24 |
6.00 |
| 6 |
Grishaeva, Maria A (11)
|
PVNCAP |
38.55 |
42.77 |
4.22 |
| 7 |
Fredericks, Sophie C (10)
|
PVMACH |
38.71 |
43.53 |
4.82 |
| 8 |
Fleming, Sharli A (11)
|
PVMACH |
38.83 |
45.13 |
6.30 |
| 9 |
Eziemefe, Adaora I (12)
|
PVMACH |
41.08 |
46.07 |
4.99 |
| 10 |
Stevens, Eliza P (12)
|
PVNCAP |
39.00 |
46.99 |
7.99 |
| 11 |
Heath, Samantha A (12)
|
PVNCAP |
42.39 |
47.37 |
4.98 |
| 12 |
Miller, Olivia L (12)
|
PVNCAP |
46.07 |
48.96 |
2.89 |
|
Enugu, Tanishka (12)
|
PVNCAP |
43.71 |
NS |
- |
|
Waugh, Olivia F (12)
|
PVMACH |
38.78 |
NS |
- |
|
Stukus, Aleena J (12)
|
PVMACH |
34.82 |
NS |
- |
|
Sargent, Macy K (11)
|
PVNCAP |
49.08 |
NS |
- |
|
Yetter, Felicity C (12)
|
PVNCAP |
31.76 |
NS |
- |