| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hosch, Lindsey N (14)
|
NTESP |
2:44.50 |
2:55.44 |
10.94 |
| 2 |
Little, Sarah A (14)
|
VATIDE |
3:01.77 |
3:01.30 |
-0.47 |
| 3 |
McCoy, Kassy R (14)
|
VATIDE |
3:15.10 |
3:04.22 |
-10.88 |
| 4 |
Fuhs, Emma K (14)
|
VATIDE |
3:09.90 |
3:05.13 |
-4.77 |
| 5 |
James, Kelsey M (14)
|
VATIDE |
3:16.54 |
3:07.98 |
-8.56 |
| 6 |
Schubert, Sydney G (14)
|
VATIDE |
3:13.15 |
3:08.25 |
-4.90 |
| 7 |
Rooks, Paige E (14)
|
VATIDE |
3:22.34 |
3:09.38 |
-12.96 |
| 8 |
Townsend, Ashliegh c (14)
|
MDHFY |
|
3:17.68 |
- |
| 9 |
McKee, Marin C (13)
|
VATIDE |
|
3:21.68 |
- |
| 10 |
Vargas, Alyssa G (13)
|
VATIDE |
|
3:22.13 |
- |
| 11 |
Knoop, Autumn E (13)
|
VATIDE |
|
3:25.18 |
- |
| 12 |
Gill, Virginia A (14)
|
VATIDE |
3:22.17 |
3:25.32 |
3.15 |
| 13 |
Crawley, Lillian N (13)
|
VATIDE |
3:32.86 |
3:28.81 |
-4.05 |
| 14 |
Woellhof, Kaidnce K (13)
|
VATIDE |
3:55.84 |
3:30.81 |
-25.03 |
| 15 |
Stidham, Catherine E (14)
|
NTESP |
|
3:35.05 |
- |
| 16 |
Mohorn, Emilia A (14)
|
VATIDE |
|
3:42.72 |
- |
| 17 |
DeMieri, Emily N (14)
|
VATIDE |
3:51.75 |
3:48.20 |
-3.55 |
| 18 |
Saddler, Haylee T (13)
|
VATIDE |
|
3:52.61 |
- |
| 19 |
Shahan, Rebecca (13)
|
MDHFY |
3:56.65 |
4:03.51 |
6.86 |
|
Stuckert, Audrey L (13)
|
NTESP |
|
DQ |
- |
|
Shinn, Alyssa R (13)
|
VAODAC |
|
NS |
- |