| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beeson, Hadley C (14)
|
NTESP |
2:32.02 |
2:35.31 |
3.29 |
| 2 |
James, Kelsey M (14)
|
VATIDE |
2:44.62 |
2:39.95 |
-4.67 |
| 3 |
Beeson, Abigail D (13)
|
NTESP |
2:47.54 |
2:40.15 |
-7.39 |
| 4 |
Gill, Virginia A (14)
|
VATIDE |
2:45.23 |
2:48.44 |
3.21 |
| 5 |
Woellhof, Kaidnce K (13)
|
VATIDE |
2:49.45 |
2:50.74 |
1.29 |
| 6 |
Moseley, Aileen C (13)
|
NTESP |
|
2:58.93 |
- |
| 7 |
Schubert, Sydney G (14)
|
VATIDE |
|
2:59.17 |
- |
| 8 |
Robertson, Alexis E (14)
|
MDHFY |
2:54.28 |
2:59.64 |
5.36 |
| 9 |
Townsend, Ashliegh c (14)
|
MDHFY |
2:51.47 |
3:00.23 |
8.76 |
| 10 |
Vargas, Alyssa G (13)
|
VATIDE |
2:58.53 |
3:02.25 |
3.72 |
| 11 |
Crawley, Lillian N (13)
|
VATIDE |
|
3:03.46 |
- |
| 12 |
Tadder, Shannon A (13)
|
VATIDE |
3:08.46 |
3:05.49 |
-2.97 |
| 13 |
Eberle, Zoe B (13)
|
VATIDE |
|
3:12.85 |
- |
| 14 |
Mohorn, Emilia A (14)
|
VATIDE |
3:26.21 |
3:12.86 |
-13.35 |
| 15 |
DeMieri, Emily N (14)
|
VATIDE |
3:09.98 |
3:13.53 |
3.55 |
| 16 |
Pogorzelski, Megan G (13)
|
VATIDE |
|
3:14.96 |
- |
| 17 |
Ryel, Alexandrea L (13)
|
VATIDE |
|
3:18.28 |
- |
| 18 |
Agarwal, Meeta (13)
|
MDHFY |
|
3:20.84 |
- |
| 19 |
Shahan, Rebecca (13)
|
MDHFY |
3:40.87 |
3:44.31 |
3.44 |
| 20 |
Yemelianov, Anna (14)
|
VATIDE |
3:53.04 |
3:49.22 |
-3.82 |
|
Shinn, Alyssa R (13)
|
VAODAC |
|
NS |
- |