| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stelitano, Mia S (8)
|
AMKW |
51.81 |
48.70 |
-3.11 |
| 2 |
Brumbaugh, Anna E (8)
|
AMWAAC |
51.79 |
53.40 |
1.61 |
| 3 |
Guan, Fiona (8)
|
AMANSC |
53.67 |
55.21 |
1.54 |
| 4 |
Good, Annalise C (8)
|
AMHD |
1:00.09 |
1:01.33 |
1.24 |
| 5 |
Sunderlin, Charlotte R (8)
|
AMANSC |
1:13.70 |
1:03.03 |
-10.67 |
| 6 |
Donaldson, Ava J (8)
|
AMANSC |
1:11.53 |
1:06.84 |
-4.69 |
| 7 |
Dabravalskas, Jovi R (8)
|
AMSVSC |
1:24.87 |
1:10.71 |
-14.16 |
| 8 |
Neupane, Charu (8)
|
AMHD |
1:25.98 |
1:14.77 |
-11.21 |
|
Bonenberger, Sophia G (8)
|
AMPTSC |
1:15.20 |
DQ |
- |
|
Murphy, Olivia C (8)
|
AMBWA |
1:20.16 |
NS |
- |
|
Ziegler, Ella E (8)
|
AMRXA |
57.60 |
NS |
- |
|
Ziegler, Sasha E (8)
|
AMRXA |
57.15 |
NS |
- |