| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhang, Weifan (13)
|
GUHCAP |
24.44 |
24.47 |
0.03 |
| 2 |
Mayorga, Oscar A (13)
|
GUHCAP |
24.56 |
25.40 |
0.84 |
| 3 |
Shkarbul, Daniil Y (13)
|
GUCOOG |
26.31 |
26.27 |
-0.04 |
| 4 |
Xu, Arbert W (14)
|
GUSSS |
27.60 |
26.42 |
-1.18 |
| 5 |
Crudo, Gabriel V (13)
|
GUSSS |
26.57 |
26.93 |
0.36 |
| 6 |
Orellana, Alexander (14)
|
GUHCAP |
27.35 |
27.50 |
0.15 |
| 7 |
Nelson, Tyler H (13)
|
GUSHRK |
26.53 |
27.60 |
1.07 |
| 8 |
Soto, Efrain A (13)
|
GUCOOG |
26.97 |
28.05 |
1.08 |
| 9 |
Rockett, Evan H (13)
|
GUSHRK |
28.33 |
28.35 |
0.02 |
| 10 |
Mcdermott, Aidan M (14)
|
GUSSS |
31.24 |
28.55 |
-2.69 |
| 11 |
Galvan, Xavier (13)
|
GUBLST |
28.28 |
28.68 |
0.40 |
| 12 |
Cousin, Isaac I (13)
|
GUHCAP |
29.27 |
28.97 |
-0.30 |
| 13 |
Charles, Noah B (13)
|
GUHCAP |
29.24 |
28.99 |
-0.25 |
| 14 |
LeCompte, Luke H (13)
|
GUSHRK |
30.28 |
29.60 |
-0.68 |
| 15 |
Szklaruk-Traipe, David I (14)
|
GUSHRK |
29.01 |
29.91 |
0.90 |
| 16 |
Rodriguez, Pedro E (14)
|
GUHCAP |
30.81 |
30.60 |
-0.21 |
| 17 |
Shelton, Nathanael A (13)
|
GUHCAP |
31.70 |
32.04 |
0.34 |
| 18 |
Saul, James L (13)
|
GUSHRK |
53.87 |
33.60 |
-20.27 |
| 19 |
Royal, Blaine A (13)
|
GUBLST |
32.88 |
33.79 |
0.91 |
| 20 |
Garcia, Luis L (13)
|
GUHCAP |
35.49 |
36.56 |
1.07 |
| 21 |
Curley, Aidan M (14)
|
GUSSS |
40.14 |
37.36 |
-2.78 |
|
Schwartzenburg, Luke D (14)
|
GUCOOG |
24.42 |
NS |
- |
|
Charles, David L (13)
|
GUHCAP |
26.62 |
NS |
- |
|
Garrett, Bryce P (13)
|
GUBLST |
30.06 |
NS |
- |