| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Jay-Colin S (12)
|
GULAP |
27.29 |
27.82 |
0.53 |
| 2 |
Grolemund, Liam J (12)
|
GUSSS |
28.24 |
28.59 |
0.35 |
| 3 |
de Zwart, Jamie W (11)
|
GUSSS |
29.42 |
28.69 |
-0.73 |
| 4 |
Butz, Jason M (12)
|
GUCOOG |
27.99 |
29.04 |
1.05 |
| 5 |
Tighe, Holden M (11)
|
GUSSS |
31.61 |
29.83 |
-1.78 |
| 6 |
Soto, Eduardo A (11)
|
GUCOOG |
30.87 |
30.32 |
-0.55 |
| 7 |
Barrera, Juan S (12)
|
GUHCAP |
32.28 |
31.04 |
-1.24 |
| 8 |
Paelicke, Ian H (12)
|
GUSSS |
31.29 |
31.51 |
0.22 |
| 9 |
Treble, James D (11)
|
GUTCAP |
31.27 |
32.58 |
1.31 |
| 10 |
Zhao, Pengbo (12)
|
GUHCAP |
35.02 |
32.88 |
-2.14 |
| 11 |
Macedo, Antonio D (11)
|
GUHCAP |
31.81 |
32.98 |
1.17 |
| 12 |
Cruz, Andres (11)
|
GUHCAP |
33.29 |
33.59 |
0.30 |
| 13 |
D'Onofrio, Cole (12)
|
GUSSS |
32.78 |
33.70 |
0.92 |
| 14 |
Leslie, Hayden V (12)
|
GUSSS |
34.92 |
34.25 |
-0.67 |
| 15 |
Morales, Sebastian A (12)
|
GUHCAP |
33.79 |
34.27 |
0.48 |
| 16 |
Stratton, Mac D (11)
|
GUCOOG |
33.42 |
34.39 |
0.97 |
| 17 |
Foster, Christopher W (12)
|
GUSSS |
35.59 |
34.55 |
-1.04 |
| 18 |
Sweeney, Owen P (12)
|
GUCOOG |
32.79 |
35.97 |
3.18 |
| 19 |
Melo, Edgar A (11)
|
GUHCAP |
35.41 |
36.67 |
1.26 |
| 20 |
Grolemund, Samuel W (11)
|
GUSSS |
35.57 |
36.76 |
1.19 |
| 21 |
Machol, Gur (12)
|
GUCOOG |
39.48 |
39.82 |
0.34 |
| 22 |
Gokce, Eral M (11)
|
GUSSS |
44.22 |
41.54 |
-2.68 |
| 23 |
Mounts, Christian M (11)
|
GUTCAP |
41.88 |
43.10 |
1.22 |
| 24 |
De Los Angeles, Sebastian (11)
|
GUCOOG |
41.58 |
44.28 |
2.70 |
| 25 |
Anderson, Kai A (11)
|
GUSSS |
45.49 |
46.13 |
0.64 |
|
Elwassimy, Malek M (12)
|
GUSHRK |
|
NS |
- |
|
Ganapathy, Auyush (11)
|
GUSHRK |
45.64 |
NS |
- |