| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hui, Rachel W (9)
|
MRLIE |
32.47 |
31.69 |
-0.78 |
| 2 |
Williamson, Blessing S (9)
|
MRLIE |
33.41 |
31.79 |
-1.62 |
| 3 |
Wong, Cailin (9)
|
MRNYCC |
34.71 |
33.35 |
-1.36 |
| 4 |
Jiang, Lori (9)
|
MRWEST |
33.59 |
33.65 |
0.06 |
| 5 |
Riva, Isabella M (9)
|
MRJCCT |
34.44 |
33.91 |
-0.53 |
| 6 |
Torres, Sofia R (9)
|
MRWEST |
35.08 |
34.27 |
-0.81 |
| 7 |
Crane, Naomi C (9)
|
MRIA |
35.17 |
34.65 |
-0.52 |
| 8 |
Johnson, Evangeline N (9)
|
MRAGUA |
34.87 |
34.76 |
-0.11 |
| 8 |
Lee, Yuna (9)
|
MRLIAC |
35.89 |
34.76 |
-1.13 |
| 10 |
Arciniegas, Alessandra (9)
|
MRCOND |
34.75 |
34.82 |
0.07 |
| 11 |
Gutierrez, Sophia E (9)
|
MRGAEL |
35.92 |
34.98 |
-0.94 |
| 12 |
Briceno, Isabella (9)
|
MRNYSA |
36.07 |
35.02 |
-1.05 |
| 13 |
Dojillo, Chloe (9)
|
MRUN |
36.10 |
35.29 |
-0.81 |
| 14 |
Kang, Sunny (9)
|
MRWEST |
36.06 |
35.62 |
-0.44 |
| 15 |
Spencer-Edwards, Lauren A (8)
|
MRUN |
35.81 |
36.30 |
0.49 |
| 16 |
Scheurer, Sydney B (9)
|
MRLIE |
35.62 |
38.47 |
2.85 |