| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeGuenther, Emory B (11)
|
VANOVA |
31.62 |
29.82 |
-1.80 |
| 2 |
Koroma, Sophie I (11)
|
PVNCAP |
31.96 |
31.43 |
-0.53 |
| 3 |
Connor, Kaitlin G (11)
|
MRLIAC |
32.58 |
31.62 |
-0.96 |
| 4 |
Callihan, Catherine B (11)
|
VANOVA |
34.23 |
31.71 |
-2.52 |
| 5 |
Tittermary, Katherine J (11)
|
VANOVA |
34.18 |
32.10 |
-2.08 |
| 6 |
Wu, Natalie (11)
|
MRLIAC |
34.30 |
32.58 |
-1.72 |
| 7 |
Mulkey, Anne M (11)
|
PVNCAP |
33.32 |
32.73 |
-0.59 |
| 8 |
Lacy, Katherine S (11)
|
VANOVA |
33.26 |
33.21 |
-0.05 |
| 9 |
So, Leni A (11)
|
MRLIAC |
33.84 |
33.28 |
-0.56 |
| 10 |
Swezey, Charlotte E (11)
|
PVNCAP |
34.67 |
33.70 |
-0.97 |
| 11 |
Hayward, Audrey I (11)
|
PVNCAP |
34.07 |
33.82 |
-0.25 |
| 12 |
Doran, Molly K (11)
|
MRLIAC |
36.38 |
34.76 |
-1.62 |