| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Austin, Thaddeus E (12)
|
NCEAC |
35.75 |
35.17 |
-0.58 |
| 2 |
Conklin, Brandt C (12)
|
NCSFSC |
36.98 |
36.73 |
-0.25 |
| 3 |
Burton, Bence W (12)
|
NCEAC |
36.07 |
37.01 |
0.94 |
| 4 |
Niebauer, Drew E (12)
|
SEBSC |
37.53 |
37.96 |
0.43 |
| 5 |
Ryan, Braden (12)
|
NCTEAM |
37.53 |
38.23 |
0.70 |
| 6 |
Burton, Tyler A (11)
|
NCEAC |
39.49 |
38.77 |
-0.72 |
| 7 |
Reiniche, Ryan J (12)
|
NCWOW |
36.90 |
39.33 |
2.43 |
| 8 |
Morgan, Noah S (11)
|
NCSFSC |
52.09 |
41.70 |
-10.39 |
| 9 |
Hoskins IV, Robert E (11)
|
NCEAC |
|
42.20 |
- |
| 10 |
Lipscomb, Michael E (11)
|
SEBSC |
40.39 |
42.37 |
1.98 |
| 11 |
Popescu, Jonathan D (12)
|
NCHAC |
44.83 |
43.68 |
-1.15 |
| 12 |
Carnahan, Declan A (11)
|
NCHAC |
45.99 |
46.45 |
0.46 |
| 13 |
Vogt, Jonathan M (11)
|
NCEAC |
45.00 |
47.28 |
2.28 |
| 14 |
Eisele, Andrew M (11)
|
VAUN-3 |
49.22 |
48.42 |
-0.80 |
| 15 |
Neighbors, Arthur J (11)
|
NCSFSC |
46.68 |
49.26 |
2.58 |
| 16 |
Tranel, Taylon K (11)
|
NCNEAL |
48.66 |
50.96 |
2.30 |
| 17 |
Tompkins, Jonathan H (11)
|
NCWOW |
51.22 |
53.04 |
1.82 |
|
Schmitz, Samuel M (11)
|
NCNEAL |
58.00 |
NS |
- |
|
Sequeiros, Sebastian (11)
|
MRNYSA |
39.36 |
NS |
- |
|
Mochalov, Daniil L (11)
|
VADYST |
|
NS |
- |