| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cukovecki, Jaxxon A (8)
|
OHCY |
51.70 |
46.74 |
-4.96 |
| 2 |
McClain, Caiden H (8)
|
OHAMFY |
51.21 |
47.34 |
-3.87 |
| 3 |
Hoge, Connor A (8)
|
OHAMFY |
52.40 |
49.48 |
-2.92 |
| 13 |
Simmons, Caleb R (8)
|
OHBASH |
|
1:00.71 |
- |
| 16 |
Dwenger, Jaxsyn T (8)
|
OHAMFY |
|
1:04.02 |
- |
| 17 |
Huelskamp, Dylan W (7)
|
OHAMFY |
|
1:06.29 |
- |
| 18 |
Li, Zachary W (7)
|
OHLAKY |
|
1:09.30 |
- |
| 22 |
Badia, Maxwell L (8)
|
OHLAKY |
1:25.04 |
1:13.80 |
-11.24 |
| 23 |
Retzler, Benjamin R (8)
|
OHBASH |
1:20.74 |
1:13.83 |
-6.91 |
| 25 |
Jansen, Oliver N (6)
|
OHCY |
1:27.09 |
1:15.36 |
-11.73 |
| 27 |
Aquilia, Chase M (7)
|
OHCY |
|
1:27.61 |
- |
| 28 |
Shane, Ethan O (8)
|
OHHHY |
|
1:39.98 |
- |
|
Rottmayer, Benjamin M (8)
|
OHCFY |
|
DQ |
- |
|
Van Schoyck, Grayden C (6)
|
OHCY |
|
DQ |
- |
|
Hao, Cody B (7)
|
OHLAKY |
|
DQ |
- |