| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kissling, Abigail M (10)
|
MAGMAC |
39.73 |
41.43 |
1.70 |
| 2 |
Kesterson, Avery B (10)
|
MAHAC |
47.29 |
46.38 |
-0.91 |
| 3 |
James, Grace A (10)
|
MAUN-0 |
48.95 |
47.64 |
-1.31 |
| 4 |
Darowish, Maya J (10)
|
MAHAC |
50.00 |
49.04 |
-0.96 |
| 5 |
Zeigler, Isabella J (10)
|
MACYA |
51.00 |
49.18 |
-1.82 |
| 6 |
Reeves, Leah M (10)
|
MAKA |
51.67 |
50.00 |
-1.67 |
| 7 |
Steinkirchner, Kaitlin P (10)
|
MACYA |
57.66 |
56.69 |
-0.97 |
| 8 |
Virabyan, Angelika J (10)
|
MAHAC |
59.49 |
59.58 |
0.09 |
| 9 |
Mccauley, Kinsey G (10)
|
MACYA |
1:17.13 |
1:14.75 |
-2.38 |
| 10 |
Bhonde, Jheel P (10)
|
MACYA |
1:12.75 |
1:14.82 |
2.07 |
|
Foley, Lily E (10)
|
MAHAC |
54.31 |
DQ |
- |
|
Bistline, Rebecca R (10)
|
MASHIP |
|
DQ |
- |