| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kaplan, Nick B (8)
|
MAVSS |
52.71 |
46.17 |
-6.54 |
| 2 |
Wilson, Gannon D (8)
|
MAUN! |
47.54 |
46.51 |
-1.03 |
| 3 |
Stavchanskiy, Brandon (8)
|
MAVSS |
50.70 |
47.96 |
-2.74 |
| 4 |
Agnello, Andraye J (8)
|
MAUN! |
51.48 |
50.26 |
-1.22 |
| 5 |
Sprang, Brayden A (8)
|
MAGPAC |
59.05 |
57.45 |
-1.60 |
| 6 |
Silipino, Alexander J (8)
|
MASTRM |
54.77 |
57.93 |
3.16 |
| 7 |
Kurian, Matthew J (8)
|
MAGPAC |
57.78 |
58.46 |
0.68 |
| 8 |
Jang, Woori E (8)
|
MAPCAC |
|
58.85 |
- |
| 9 |
Donovan, Ethan J (8)
|
MAUN! |
1:05.68 |
1:03.50 |
-2.18 |
| 10 |
Balog, Colton (8)
|
MAT85 |
1:07.27 |
1:03.73 |
-3.54 |
| 11 |
Christenson-Aurand, Fintan J (8)
|
MASTRM |
|
1:21.36 |
- |
|
Jones, Bryce C (8)
|
MAGPAC |
|
DQ |
- |
|
Berthelotte, Liam N (8)
|
MASTRM |
|
DQ |
- |
|
Schroeder, Emmett L (8)
|
MAECCC |
1:05.02 |
NS |
- |
|
Long, Francis A (8)
|
MAFSA |
52.98 |
NS |
- |