| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Brien, Charlie G (10)
|
MRTS |
33.33 |
32.87 |
-0.46 |
| 2 |
Mitsuhashi, Yuuki (9)
|
MRYMID |
37.08 |
36.19 |
-0.89 |
| 3 |
Montagnino, Tyler F (10)
|
MRFA |
34.62 |
36.30 |
1.68 |
| 4 |
Shemetov, Ivan T (10)
|
MRFA |
39.13 |
38.47 |
-0.66 |
| 5 |
O'Shea, Aiden P (10)
|
MRLBA |
39.93 |
39.29 |
-0.64 |
| 6 |
Deangelis, Nicholas A (9)
|
MRLBA |
40.11 |
39.59 |
-0.52 |
| 7 |
Ruffy, Rhyley X (10)
|
MRNYSA |
44.75 |
43.35 |
-1.40 |
| 8 |
Unger, Ethan S (10)
|
MRFA |
52.62 |
44.04 |
-8.58 |
| 9 |
Mummery, Conor J (10)
|
MRNBS |
45.91 |
44.56 |
-1.35 |
| 10 |
Lieberman, Sawyer J (10)
|
MRLBA |
42.53 |
46.67 |
4.14 |
| 11 |
Lazatin, Thomas J (9)
|
MRVAC |
55.67 |
47.01 |
-8.66 |
| 12 |
Howard, Sam W (10)
|
MRRFAC |
49.52 |
47.28 |
-2.24 |
| 13 |
Muety, Shaun W (9)
|
MRLBA |
52.59 |
47.32 |
-5.27 |
| 14 |
Abreu, Alex C (9)
|
MRFA |
45.42 |
47.60 |
2.18 |
| 15 |
Chaplin, Connor M (10)
|
MRFA |
45.28 |
47.72 |
2.44 |
| 16 |
Tennant, Jackson M (8)
|
MRNBS |
50.09 |
47.87 |
-2.22 |
| 17 |
Townsend, Lukas J (9)
|
MRNBS |
49.06 |
52.74 |
3.68 |
| 18 |
Shaw, Jaden K (10)
|
MRMWDA |
52.63 |
53.94 |
1.31 |
| 19 |
Felker, Christopher D (10)
|
MRMWDA |
53.26 |
55.21 |
1.95 |
| 20 |
Gonzales, Justin W (10)
|
MRWSSC |
54.95 |
56.36 |
1.41 |
| 21 |
Banavara, Aditya D (8)
|
MRWSSC |
1:04.30 |
57.83 |
-6.47 |
| 22 |
Freer, Evan N (9)
|
MRVAC |
1:05.45 |
58.25 |
-7.20 |
| 23 |
Tuttle, Ryder J (10)
|
MRNBS |
|
1:00.74 |
- |
|
Vega, Isidro R (9)
|
MRRFAC |
49.22 |
DQ |
- |
|
Scalia, Pietro N (9)
|
MRWSSC |
|
DQ |
- |