| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kitlowski, Kara O (8)
|
MRLIAC |
39.62 |
36.80 |
-2.82 |
| 2 |
McMahon, Reilly E (8)
|
MRWISC |
38.20 |
38.59 |
0.39 |
| 3 |
Hu, Allison (8)
|
MRLIAC |
40.40 |
38.82 |
-1.58 |
| 4 |
Gans, Josie L (8)
|
MRLIAC |
46.09 |
40.24 |
-5.85 |
| 5 |
Hoye, Mea (7)
|
MRLGAC |
42.63 |
40.37 |
-2.26 |
| 6 |
Coratti, Lilian M (7)
|
MRLIAC |
55.00 |
40.96 |
-14.04 |
| 7 |
Tran, Brianna (8)
|
MRST |
56.54 |
41.04 |
-15.50 |
| 8 |
Chambless, Virginia S (8)
|
MRLIAC |
1:10.32 |
41.48 |
-28.84 |
| 9 |
Udomsilp, Natalie (8)
|
MRLGAC |
44.02 |
41.54 |
-2.48 |
| 10 |
Barrera, Daniella (8)
|
MRLGAC |
|
42.17 |
- |
| 11 |
Zayas, Leandra (8)
|
MDBCSC |
41.11 |
42.38 |
1.27 |
| 12 |
DiBlasi, Elizabeth K (8)
|
MRLIAC |
45.12 |
42.69 |
-2.43 |
| 13 |
Diaz, Isabella (8)
|
NJUN |
40.83 |
43.96 |
3.13 |
| 14 |
Fraczek, Matylda (8)
|
MRLGAC |
46.40 |
44.80 |
-1.60 |
| 15 |
Phillips, Erin B (7)
|
MRLIAC |
45.69 |
46.59 |
0.90 |
| 16 |
Sacoulas, Ava D (8)
|
MRLIAC |
46.80 |
46.99 |
0.19 |
| 17 |
Liang, YuHong (7)
|
MRLIAC |
1:00.00 |
47.45 |
-12.55 |
| 18 |
Brink, Sarah R (7)
|
MRWISC |
58.38 |
51.09 |
-7.29 |
| 19 |
Tuen, Kristen (7)
|
MRST |
57.42 |
51.14 |
-6.28 |
| 20 |
Cooper, Madeline M (7)
|
MRWISC |
1:15.99 |
56.96 |
-19.03 |
| 21 |
Riley, Candice M (8)
|
MDBCSC |
1:04.17 |
57.51 |
-6.66 |
| 22 |
Davis, Adanyah G (8)
|
MRNFS |
|
1:01.67 |
- |
| 23 |
Stanton-Barnett, Baylee M (7)
|
MRWISC |
1:04.28 |
1:01.82 |
-2.46 |
| 24 |
Cullen, Moxie J (5)
|
MRWISC |
1:20.99 |
1:10.38 |
-10.61 |
| 25 |
Allen, Christina (7)
|
MRNFS |
|
1:20.73 |
- |
|
Perr, Abbie (6)
|
MRLGAC |
|
NS |
- |
|
Nguyen, Olivia B (8)
|
MRSSC |
41.46 |
NS |
- |