| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vladimiroff, Daniel (9)
|
PVMACH |
39.06 |
36.10 |
-2.96 |
| 2 |
Raybon, Xavier L (9)
|
PVMACH |
39.19 |
36.73 |
-2.46 |
| 3 |
Buczek, Anabel H (9)
|
PVMACH |
|
39.04 |
- |
| 4 |
Wu, Gabriel B (9)
|
PVMACH |
46.58 |
39.37 |
-7.21 |
| 5 |
Rivera-Castro, Noelle I (9)
|
PVMACH |
52.72 |
40.50 |
-12.22 |
| 6 |
Lay, Kamryn J (9)
|
PVMACH |
44.42 |
41.20 |
-3.22 |
| 7 |
Dalton, Umi J (9)
|
PVMACH |
47.14 |
43.39 |
-3.75 |
| 8 |
Laughlin, Bridget S (9)
|
PVMACH |
|
43.57 |
- |
| 9 |
Pezzimenti, Natalia M (9)
|
PVMACH |
57.60 |
44.09 |
-13.51 |
| 10 |
Radio, Lucy A (9)
|
PVMACH |
|
44.23 |
- |
| 11 |
Yu, Felix P (9)
|
PVMACH |
51.56 |
44.76 |
-6.80 |
| 12 |
Lasut, Claire I (9)
|
PVMACH |
53.67 |
47.35 |
-6.32 |
| 13 |
Engledow, Alice B (9)
|
PVMACH |
|
47.83 |
- |
| 14 |
Peterson, Emma R (9)
|
PVMACH |
|
51.67 |
- |
| 15 |
Roberts Nijim, Sami J (9)
|
PVMACH |
57.63 |
53.66 |
-3.97 |
| 16 |
Onerci, Gizem (9)
|
PVMACH |
|
57.06 |
- |
|
Zhao, Lucas X (9)
|
PVMACH |
|
DQ |
- |
|
Pai, Divya I (9)
|
PVMACH |
|
DQ |
- |
|
Steele, Georgia K (9)
|
PVMACH |
|
NS |
- |
|
Cherryholmes, Caleb J (9)
|
PVMACH |
|
NS |
- |